Evaluation of the effect of enriched hydrogen saline solution on distant organ (lung) damage in skeletal muscle ischemia reperfusion in rats

Introduction Ischemia-reperfusion (IR) injury is a major concern that frequently occurs during vascular surgeries. Hydrogen-rich saline (HRS) solution exhibits antioxidant and anti-inflammatory properties. This study aimed to examine the effects of HRS applied before ischemia in the lungs of rats us...

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Published in:Science progress (1916) 2024-04, Vol.107 (2), p.368504241257060
Main Authors: Özer, Abdullah, Erel, Selin, Küçük, Ayşegül, Demirtaş, Hüseyin, Sezen, Şaban Cem, Boyunağa, Hakan, Oktar, Gürsel Levent, Arslan, Mustafa
Format: Article
Language:English
Subjects:
Alveoli
Animal models
Animals
Anti-inflammatory agents
Aorta
Damage assessment
Histopathology
Hydrogen
Hydrogen - administration & dosage
Hydrogen - pharmacology
Infiltration
Injuries
Ischemia
Leukocytes (neutrophilic)
Lipid peroxidation
Lipids
Lung - blood supply
Lung - drug effects
Lung - metabolism
Lung - pathology
Lung Injury - drug therapy
Lung Injury - pathology
Lungs
Male
Malondialdehyde - metabolism
Medicine & Health Sciences
Microvasculature
Muscle, Skeletal - blood supply
Muscle, Skeletal - drug effects
Muscle, Skeletal - metabolism
Muscle, Skeletal - pathology
Muscles
Musculoskeletal system
Neutrophils
Oxidative stress
Peroxidation
Rats
Rats, Wistar
Reperfusion
Reperfusion Injury - drug therapy
Reperfusion Injury - pathology
Saline Solution - administration & dosage
Saline Solution - chemistry
Saline Solution - pharmacology
Saline solutions
Skeletal muscle
Statistical analysis
Thickness
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Summary:Introduction Ischemia-reperfusion (IR) injury is a major concern that frequently occurs during vascular surgeries. Hydrogen-rich saline (HRS) solution exhibits antioxidant and anti-inflammatory properties. This study aimed to examine the effects of HRS applied before ischemia in the lungs of rats using a lower extremity IR model. Material and Methods After approval was obtained from the ethics committee, 18 male Wistar albino rats weighing 250–280 g were randomly divided into three groups: control (C), IR and IR-HRS. In the IR and IR-HRS groups, an atraumatic microvascular clamp was used to clamp the infrarenal abdominal aorta, and skeletal muscle ischemia was induced. After 120 min, the clamp was removed, and reperfusion was achieved for 120 min. In the IR-HRS group, HRS was administered intraperitoneally 30 min before the procedure. Lung tissue samples were examined under a light microscope and stained with hematoxylin-eosin (H&E). Malondialdehyde (MDA) levels, total sulfhydryl (SH) levels, and histopathological parameters were evaluated in the tissue samples. Results MDA and total SH levels were significantly higher in the IR group than in the control group (p 
ISSN:0036-8504
2047-7163
2047-7163
DOI:10.1177/00368504241257060