Effect of BCG stimulus on proinflammatory cytokine production in laryngeal cancer

Background Evaluate the production of TNF and IL-6 in the supernatant of peripheral blood mononuclear cell (PBMC) cultures of patients with supraglottic laryngeal cancer before and after surgical treatment. Materials and methods Adherent cell cultures were stimulated with LPS and BCG. Fourteen patie...

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Bibliographic Details
Published in:Cancer Immunology, Immunotherapy Immunotherapy, 2009-01, Vol.58 (1), p.25-29
Main Authors: Conti-Freitas, Luiz Carlos, Foss-Freitas, Maria Cristina, Mamede, Rui Celso Martins, Foss, Norma Tiraboschi
Format: Article
Language:English
Subjects:
Adult
Aged
Antineoplastic agents
Bacterial diseases
BCG Vaccine - immunology
BCG Vaccine - therapeutic use
Biological and medical sciences
Cancer Research
Carcinoma - drug therapy
Cell culture
Cytokines
Cytokines - biosynthesis
Cytokines - blood
Cytokines - immunology
Female
Human bacterial diseases
Humans
Immunology
Immunotherapy
Infectious diseases
Inflammation
Interleukin-6 - biosynthesis
Interleukin-6 - blood
Interleukin-6 - immunology
Laryngeal cancer
Laryngeal Neoplasms - drug therapy
Laryngeal Neoplasms - immunology
Lymphocytes
Male
Medical sciences
Medicine
Medicine & Public Health
Middle Aged
Neoplasm Staging
Oncology
Original
Original Article
Otorhinolaryngology. Stomatology
Patients
Pharmacology. Drug treatments
Postoperative period
Smoking
Surgery
Tuberculosis and atypical mycobacterial infections
Tumor Necrosis Factor-alpha - biosynthesis
Tumor Necrosis Factor-alpha - blood
Tumor Necrosis Factor-alpha - immunology
Tumor necrosis factor-TNF
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
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Summary:Background Evaluate the production of TNF and IL-6 in the supernatant of peripheral blood mononuclear cell (PBMC) cultures of patients with supraglottic laryngeal cancer before and after surgical treatment. Materials and methods Adherent cell cultures were stimulated with LPS and BCG. Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cytokine concentration was determined by ELISA in supernatants of mononuclear cell cultures. Results In non-stimulated cultures, lower TNF cytokine levels were detected during the late postoperative (LP) period compared to control ( P  = 0.02). LP TNF and IL-6 levels were high in cultures stimulated with LPS compared with the preoperative period (PREOP) ( P  = 0.007; P  = 0.008, respectively). Stimulation with BCG led to increased levels of TNF and IL-6 during the LP period compared to control ( P  = 0.001; P  = 0.04, respectively). Conclusion BCG is able to modulate the immune response of patients with advanced supraglottic laryngeal cancer in vitro, increasing the secretion of TNF and IL-6 by macrophages during the postoperative period.
ISSN:0340-7004
1432-0851
DOI:10.1007/s00262-008-0520-7