Nuclear protein‐1 is the common link for pathways activated by aging and obesity in chondrocytes: A potential therapeutic target for osteoarthritis

Pathways leading to osteoarthritis (OA) are diverse depending on the risk factors involved; thus, developing OA therapeutics has been challenging. Here we report that nuclear protein‐1 (Nupr1), a stress‐inducible protein/transcription factor, is activated by pathways associated with obesity and agin...

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Published in:The FASEB journal 2023-09, Vol.37 (9), p.e23133-n/a
Main Authors: Tan, Li, Armstrong, Alexandra R., Rosas, Samuel, Patel, Chirayu M., Vander Wiele, Sabrina S., Willey, Jeffrey S., Carlson, Cathy S., Yammani, Raghunatha R.
Format: Article
Language:English
Subjects:
Aging
Animals
Cartilage, Articular - metabolism
cellular stress
Chondrocytes - metabolism
Humans
Mice
Nuclear Proteins - metabolism
Nupr1
obesity
Obesity - metabolism
osteoarthritis
Osteoarthritis - metabolism
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Summary:Pathways leading to osteoarthritis (OA) are diverse depending on the risk factors involved; thus, developing OA therapeutics has been challenging. Here we report that nuclear protein‐1 (Nupr1), a stress‐inducible protein/transcription factor, is activated by pathways associated with obesity and aging in chondrocytes. Treatment of human chondrocytes with free fatty acids (palmitate and oleate; a model for high‐fat diet/obesity) induced PERK signaling and increased expression of caspase‐3, TRB3, and Nupr1. On the other hand, treatment of chondrocytes with menadione (oxidative stress inducer) induced oxidation of IRE1, activated antioxidant response (higher Nrf2 expression), and increased expression of Nupr1 and matrix metalloproteinases. Experimental OA was induced by destabilization of the medial meniscus (DMM) in the knee joints of Nupr1+/+ and Nupr1−/− mice. Loss of Nupr1 expression reduced the severity of cartilage lesions in this model. Together, our findings suggest that Nupr1 is a common factor activated by signaling pathways activated by obesity (ER stress) and age (oxidative stress) and a potential drug target for OA resulting from various risk factors. Model showing critical role of Nupr1 in obesity, aging, and injury‐linked OA pathogenesis.
ISSN:0892-6638
1530-6860
DOI:10.1096/fj.202201700RR