Temperature influences commensal-pathogen dynamics in a nasal epithelial cell co-culture model

Chronic rhinosinusitis (CRS) is an inflammatory disease of the paranasal sinuses, and microbial dysbiosis associated with CRS is thought to be a key driver of host inflammation that contributes to disease progression. is a common upper respiratory tract (URT) pathobiont associated with higher carria...

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Bibliographic Details
Published in:mSphere 2024-01, Vol.9 (1), p.e0058923-e0058923
Main Authors: Huffines, Joshua T, Boone, RaNashia L, Kiedrowski, Megan R
Format: Article
Language:English
Subjects:
Air temperature
Bacteriology
Cell culture
Chronic Disease
Clinical isolates
Coculture Techniques
Colonization
Corynebacterium
Cytotoxicity
Disease
Dysbacteriosis
Dysbiosis
Endoscopy
Environmental changes
Epithelial cells
Epithelial Cells - microbiology
Epithelium
Humans
Inflammation
Inflammatory diseases
Microscopy
Nasopharynx
Otolaryngology
Paranasal sinus
Pathogens
Pathology
Research Article
Respiratory tract
Rhinosinusitis
Sinuses
Sinusitis - microbiology
Species
Staphylococcus aureus
Surgery
Temperature
Trends
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Summary:Chronic rhinosinusitis (CRS) is an inflammatory disease of the paranasal sinuses, and microbial dysbiosis associated with CRS is thought to be a key driver of host inflammation that contributes to disease progression. is a common upper respiratory tract (URT) pathobiont associated with higher carriage rates in CRS populations, where -secreted toxins can be identified in CRS tissues. Although many genera of bacteria colonize the URT, few account for the majority of sequencing reads. These include and several species belonging to the genus , including and , which are observed at high relative abundance in the healthy URT. Studies have examined bacterial interactions between major microbionts of the URT and , but few have done so in the context of a healthy versus diseased URT environment. Here, we examine the role of temperature in commensal, pathogen, and epithelial dynamics using an air-liquid interface cell culture model mimicking the nasal epithelial environment. Healthy URT temperatures change from the nares to the nasopharynx and are increased during disease. Temperatures representative of the healthy URT increase persistence and aggregate formation of commensal , reduce growth, and lower epithelial cytotoxicity compared to higher temperatures correlating with the diseased CRS sinus. Dual-species colonization revealed species-specific interactions between species and dependent on temperature. Our findings suggest URT mucosal temperature plays a significant role in mediating polymicrobial and host-bacterial interactions that may exacerbate microbial dysbiosis in chronic URT diseases.IMPORTANCEChronic rhinosinusitis is a complex inflammatory disease with a significant healthcare burden. Although presence of and microbial dysbiosis are considered mediators of inflammation in CRS, no studies have examined the influence of temperature on interactions with the nasal epithelium and the dominant genus of the healthy URT, . Interactions between species and have been documented in several studies, but none to date have examined how environmental changes in the URT may alter their interactions with the epithelium or each other. This study utilizes a polarized epithelial cell culture model at air-liquid interface to study the colonization and spatial dynamics of and clinical isolates of from people with CRS to characterize the role temperature has in single- and dual-species dynamics on the nasal epithelium.
ISSN:2379-5042
2379-5042
DOI:10.1128/msphere.00589-23