Null mutants of a tomato Rho of plants exhibit enhanced water use efficiency without a penalty to yield

Improving water use efficiency in crops is a significant challenge as it involves balancing water transpiration and CO uptake through stomatal pores. This study investigates the role of SlROP9, a tomato Rho of Plants protein, in guard cells and its impact on plant transpiration. The results reveal t...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2024-01, Vol.121 (4), p.e2309006120
Main Authors: Puli, Mallikarjuna R, Muchoki, Purity, Yaaran, Adi, Gershon, Noga, Dalal, Ahan, Nalufunjo, Felista, Dagan, Yoav, Rosental, Leah, Abadi, Shiran, Haber, Zachary, Silva, Lucas, Brotman, Yariv, Sade, Nir, Yalovsky, Shaul
Format: Article
Language:English
Subjects:
Abscisic Acid
Arabidopsis - genetics
Biological Sciences
Carbon dioxide
Crop yield
Crops
Crops, Agricultural
Drought
Efficiency
Field tests
Guard cells
Mutants
NAD(P)H oxidase
Oxidase
Phenotypes
Plant Proteins - genetics
Plants (botany)
Reactive oxygen species
Respiratory burst oxidase
Solanum lycopersicum - genetics
Stomata
Stomatal conductance
Tomatoes
Transpiration
Water pressure
Water use
Water use efficiency
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Improving water use efficiency in crops is a significant challenge as it involves balancing water transpiration and CO uptake through stomatal pores. This study investigates the role of SlROP9, a tomato Rho of Plants protein, in guard cells and its impact on plant transpiration. The results reveal that SlROP9 null mutants exhibit reduced stomatal conductance while photosynthetic CO assimilation remains largely unaffected. Notably, there is a notable decrease in whole-plant transpiration in the mutants compared to the wild type, especially during noon hours when the water pressure deficit is high. The elevated stomatal closure observed in mutants is linked to an increase in reactive oxygen species formation. This is very likely dependent on the respiratory burst oxidase homolog (RBOH) NADPH oxidase and is not influenced by abscisic acid (ABA). Consistently, activated ROP9 can interact with RBOHB in both yeast and plants. In diverse tomato accessions, drought stress represses expression, and in stomatal guard cells, ABA suppresses ROP signaling. Therefore, the phenotype of the mutants may arise from a disruption in ROP9-regulated RBOH activity. Remarkably, large-scale field experiments demonstrate that the mutants display improved water use efficiency without compromising fruit yield. These findings provide insights into the role of ROPs in guard cells and their potential as targets for enhancing water use efficiency in crops.
ISSN:0027-8424
1091-6490
1091-6490
DOI:10.1073/pnas.2309006120