Biogenic silver nanoparticles' antibacterial activity and cytotoxicity on human hepatocarcinoma cells (Huh-7)

Exploring diverse synthetic pathways for nanomaterial synthesis has emerged as a promising direction. For example, silver nanoparticles (AgNPs) are synthesized using different approaches yielding nanomaterials with distinct morphological, physical and biological properties. Hence, the present study...

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Bibliographic Details
Published in:RSC advances 2024-01, Vol.14 (4), p.2192-224
Main Authors: Santana da Costa, Thyerre, Rodrigues da Silva, Mariana, Jerônimo Barbosa, Júlio César, Da Silva Das Neves, Uedson, de Jesus, Marcelo Bispo, Tasic, Ljubica
Format: Article
Language:English
Subjects:
Anticancer properties
Bacteria
Biocompatibility
Biological properties
Cancer
Chemical synthesis
Chemistry
Cytotoxicity
E coli
Fusarium oxysporum
Microscopy
Molecular interactions
Morphology
Nanomaterials
Nanoparticles
Scanning transmission electron microscopy
Silver
Toxicity
Zeta potential
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Summary:Exploring diverse synthetic pathways for nanomaterial synthesis has emerged as a promising direction. For example, silver nanoparticles (AgNPs) are synthesized using different approaches yielding nanomaterials with distinct morphological, physical and biological properties. Hence, the present study reports the biogenic synthesis of silver nanoparticles using the aqueous secretome of the fungus Fusarium oxysporum f. sp. cubense (AgNP@Fo) and orange peel extract (AgNP@OR). The physical and morphological properties of synthesized nanoparticles were similar, with AgNP@Fo measuring 56.43 ± 19.18 nm and AgNP@OR measuring 39.97 ± 19.72 nm in size. The zeta potentials for the nanoparticles were low, −26.8 ± 7.55 and −26.2 ± 2.87 mV for AgNP@Fo and AgNP@OR, respectively, demonstrating a similar negative charge. The spherical morphologies of both nanoparticles were evidenced by Scanning Transmission Electron Microscopy (STEM) and Atomic Force Microscopy (AFM). However, despite their similar physical and morphological properties, AgNPs demonstrated different bioactivities. We evaluated and compared the antimicrobial efficacy of these nanoparticles against a range of bacteria, such as Staphylococcus aureus , Enterococcus faecalis , Pseudomonas aeruginosa , and Escherichia coli . The AgNP@Fo showed Minimum Inhibitory Concentration (MIC) values ranging from 0.84 to 1.68 μg mL −1 and were around ten times more potent compared to AgNP@OR. The anticancer activities of both nanoparticles were investigated using human hepatocarcinoma cells (Huh-7), where AgNP@Fo exhibited around 20 times higher cytotoxicity than AgNP@OR with an IC 50 value of 0.545 μmol L −1 . Anticancer effects were demonstrated by the MTT, confirmed by the calcein-AM assay and fluorescence imaging. This study establishes solid groundwork for future exploration of molecular interactions of nanoparticles synthesized through distinct biosynthetic routes, particularly within bacterial and cancerous cell environments. This study aimed to explore the antibacterial and anticancer potential of biosynthesized silver nanoparticles and how the biosynthesis of AgNP@Bio determines their biological roles.
ISSN:2046-2069
2046-2069
DOI:10.1039/d3ra07733k