Age-related expression analysis of mouse liver nuclear protein binding to 3'-untranslated region of Period2 gene

In mammals, both circadian rhythm and aging play important roles in regulating time-dependent homeostasis. We previously discovered an age-related increase element binding protein, hnRNP A3, which binds to the 3'-untranslated region (UTR) of blood coagulation factor IX (FIX). Here, we describe...

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Bibliographic Details
Published in:The journal of physiological sciences 2015-07, Vol.65 (4), p.349-357
Main Authors: Hamada, Toshiyuki, Miyakawa, Kazuko, Kushige, Hiroko, Shibata, Shigenobu, Kurachi, Sumiko
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Aging - genetics
Aging - metabolism
Animals
Base Sequence
Binding Sites - genetics
Circadian Rhythm - genetics
Circadian Rhythm - physiology
Heterogeneous-Nuclear Ribonucleoprotein Group C - metabolism
Heterogeneous-Nuclear Ribonucleoprotein Group F-H - metabolism
Liver - metabolism
Mice
Mice, Inbred C57BL
Original Paper
Period Circadian Proteins - genetics
Protein Binding
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Summary:In mammals, both circadian rhythm and aging play important roles in regulating time-dependent homeostasis. We previously discovered an age-related increase element binding protein, hnRNP A3, which binds to the 3'-untranslated region (UTR) of blood coagulation factor IX (FIX). Here, we describe other members of this protein family, hnRNP C and hnRNP H, which bind to the 3'-UTR of the mouse circadian clock gene Period 2 (mPer2). RNA electrophoretic mobility shift assays using a (32)P-labeled Per2 RNA probe coupled with two-dimensional gel electrophoresis followed by MALDI-TOF/MS peptide mass fingerprint analysis was used to analyze these proteins. Western blotting suggested that the total expression of these proteins in mouse liver cell nuclei does not increase with age. Two-dimensional gel electrophoresis analysis of age-related protein expression showed that many isoforms of these proteins exist in the liver and that each protein exhibits a complex age-related expression pattern. These results suggest that many isoforms of proteins are regulated by different aging systems and that many age regulation systems function in the liver.
ISSN:1880-6546
1880-6562
DOI:10.1007/s12576-015-0373-8