The role of catecholaminergic neurons in the hypothalamus and medullary visceral zone in response to restraint water-immersion stress in rats

The activity of catecholaminergic neurons in the hypothalamus and the medullary visceral zone (MVZ) in rats in response to restraint water-immersion stress (RWIS) was measured by use of dual Fos and tyrosine hydroxylase (TH) immunohistochemistry. In RWIS rats Fos immunoreactive (Fos-IR) nuclei drama...

Full description

Saved in:
Bibliographic Details
Published in:The journal of physiological sciences 2011-01, Vol.61 (1), p.37-45
Main Authors: Zhao, Dong-Qin, Lu, Chang-Liang, Ai, Hong-Bin
Format: Article
Language:English
Subjects:
Adrenal Medulla - metabolism
Animals
Area Postrema - metabolism
Catecholamines - physiology
Dehydration - metabolism
Hypothalamus - cytology
Hypothalamus - metabolism
Hypothalamus - physiology
Immersion
Immunohistochemistry
Male
Medulla Oblongata - cytology
Medulla Oblongata - metabolism
Medulla Oblongata - physiology
Neurons - metabolism
Neurons - physiology
Original Paper
Paraventricular Hypothalamic Nucleus - metabolism
Proto-Oncogene Proteins c-fos - metabolism
Rats
Rats, Wistar
Solitary Nucleus - metabolism
Supraoptic Nucleus - metabolism
Tyrosine 3-Monooxygenase - metabolism
Vagus Nerve - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The activity of catecholaminergic neurons in the hypothalamus and the medullary visceral zone (MVZ) in rats in response to restraint water-immersion stress (RWIS) was measured by use of dual Fos and tyrosine hydroxylase (TH) immunohistochemistry. In RWIS rats Fos immunoreactive (Fos-IR) nuclei dramatically increased in the paraventricular nucleus (PVN), the supraoptic nucleus (SON), the dorsal motor nucleus of the vagus (DMV), the nucleus of the solitary tract (NTS), the area postrema (AP), and the ventrolateral medulla (VLM). A small number of TH-immunoreactive (TH-IR) and Fos/TH double-labeling neurons in the PVN, and their absence from the SON, were observed in both RWIS and nonstressed rats. More TH-IR neurons were observed in the MVZ of RWIS rats than in nonstressed rats. In RWIS and nonstressed rats, the percentage of Fos-IR nuclei in TH-IR neurons was 38.0 and 14.3% in the DMV, 34.4 and 9.7% in the NTS, 18.6 and 4.5% in the AP, and 45.7 and 18.9% in the VLM, respectively. In conclusion, catecholaminergic neurons in the MVZ are involved in the response to RWIS; although the PVN and SON also participate in the response to RWIS, the mechanism is not via catecholaminergic neurons.
ISSN:1880-6546
1880-6562
DOI:10.1007/s12576-010-0119-6