Cleavage kinetics of human mitochondrial RNase P and contribution of its non-nuclease subunits

Abstract RNase P is the endonuclease responsible for the 5′ processing of precursor tRNAs (pre-tRNAs). Unlike the single-subunit protein-only RNase P (PRORP) found in plants or protists, human mitochondrial RNase P is a multi-enzyme assembly that in addition to the homologous PRORP subunit comprises...

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Published in:	Nucleic acids research 2023-10, Vol.51 (19), p.10536-10550
Main Authors:	Vilardo, Elisa, Toth, Ursula, Hazisllari, Enxhi, Hartmann, Roland K, Rossmanith, Walter
Format:	Article
Language:	eng
Subjects:	Molecular Biology
Online Access:	Get full text
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Summary:	Abstract RNase P is the endonuclease responsible for the 5′ processing of precursor tRNAs (pre-tRNAs). Unlike the single-subunit protein-only RNase P (PRORP) found in plants or protists, human mitochondrial RNase P is a multi-enzyme assembly that in addition to the homologous PRORP subunit comprises a methyltransferase (TRMT10C) and a dehydrogenase (SDR5C1) subunit; these proteins, but not their enzymatic activities, are required for efficient pre-tRNA cleavage. Here we report a kinetic analysis of the cleavage reaction by human PRORP and its interplay with TRMT10C-SDR5C1 including 12 different mitochondrial pre-tRNAs. Surprisingly, we found that PRORP alone binds pre-tRNAs with nanomolar affinity and can even cleave some of them at reduced efficiency without the other subunits. Thus, the ancient binding mode, involving the tRNA elbow and PRORP’s PPR domain, appears basically retained by human PRORP, and its metallonuclease domain is in principle correctly folded and functional. Our findings support a model according to which the main function of TRMT10C-SDR5C1 is to direct PRORP’s nuclease domain to the cleavage site, thereby increasing the rate and accuracy of cleavage. This functional dependence of human PRORP on an extra tRNA-binding protein complex likely reflects an evolutionary adaptation to the erosion of canonical structural features in mitochondrial tRNAs. Graphical Abstract Graphical Abstract
ISSN:	0305-1048 1362-4962