Involvement of proliferation of atypical hepatocytes and CDT 1 in the liver cancer of rats administered the diethylnitrosamine

We have reported that extent of proliferation of atypical hepatocytes (POAH) in non-cancerous liver in hepatocellular carcinoma and chromatin licensing and DNA replication factor 1 (CDT1) are associated with postoperative recurrence. Here, we investigated whether extent of POAH and expression of CDT...

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Published in:Journal of Clinical Biochemistry and Nutrition 2023, Vol.73(2), pp.138-144
Main Authors: Ogawa, Masahiro, Masuzaki, Ryota, Kanda, Tatsuo, Matsumura, Hiroshi, Nakamura, Hitomi, Yamazaki, Motomi, Shibata, Toshikatu, Kogure, Hirofumi, Moriyama, Mitsuhiko
Format: Article
Language:English
Subjects:
Carcinogens
Chromatin
chromatin licensing and DNA replication factor 1
Diethylnitrosamine
DNA biosynthesis
Drinking water
Gene expression
Hepatocellular carcinoma
Hepatocytes
Immunohistochemistry
irregular regeneration of hepatocytes
Liver
Liver cancer
Oral administration
Original
proliferation of atypical hepatocyte
Time dependence
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Summary:We have reported that extent of proliferation of atypical hepatocytes (POAH) in non-cancerous liver in hepatocellular carcinoma and chromatin licensing and DNA replication factor 1 (CDT1) are associated with postoperative recurrence. Here, we investigated whether extent of POAH and expression of CDT1 in ‍liver are also associated with chemically induced liver cancer in ‍rats. Male Fisher strain rats were orally administered diethylnitrosamine (DEN) in their drinking water and sacrificed at 6, 8, 12, or 14 weeks after start of DEN administration. We serially monitored changes in extent of POAH, CDT1 expression by immunohistochemistry (IHC), and CDT1 mRNA expression in liver by real-time quantitative PCR. The extent of POAH in liver progressed in a time-dependent manner after start of DEN administration. CDT1 expression was higher at 8 weeks than at 6 weeks by IHC, suggesting that CDT1 expression may be a marker of POAH severity. CDT1 mRNA expression in liver was significantly higher at 12 weeks than at 6 weeks (p
ISSN:0912-0009
1880-5086
DOI:10.3164/jcbn.13-16