Neuromodulatory role of L-arginine: nitric oxide precursor against thioacetamide-induced-hepatic encephalopathy in rats via downregulation of NF-κB-mediated apoptosis

The aim of the present study was to investigate the impact of arginine (ARG), a nitric oxide (NO) precursor, on thioacetamide (TAA)-induced hepatic encephalopathy (HE) in rats by injection of TAA (100 mg/kg, i.p) three times per week for six consecutive weeks. TAA-injected rats were administered ARG...

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Bibliographic Details
Published in:Environmental science and pollution research international 2023-07, Vol.30 (35), p.84791-84804
Main Authors: Sedik, Ahmed A., Hassan, Azza, Saleh, Dalia O.
Format: Article
Language:English
Subjects:
Alanine
Alanine transaminase
Alkaline phosphatase
Ammonia
Animals
Apoptosis
Aquatic Pollution
Arginine
Arginine - adverse effects
Arginine - metabolism
Aspartate transaminase
Atmospheric Protection/Air Quality Control/Air Pollution
Bilirubin
Biomarkers
Brain
Caspase-3
Cerebellum
Complications
Down-Regulation
Earth and Environmental Science
Ecotoxicology
Encephalopathy
Environment
Environmental Chemistry
Environmental Health
Environmental science
Hepatic encephalopathy
Hepatic Encephalopathy - chemically induced
Hyperammonemia
Immunology
Inflammation
Liver
Liver - metabolism
Locomotor activity
Motor skill learning
Motor task performance
Neuroimaging
NF-kappa B - metabolism
NF-κB protein
Nitric oxide
Nitric Oxide - metabolism
Oxidative Stress
Precursors
Rats
Research Article
Restoration
Thioacetamide
Thioacetamide - toxicity
Waste Water Technology
Water Management
Water Pollution Control
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Summary:The aim of the present study was to investigate the impact of arginine (ARG), a nitric oxide (NO) precursor, on thioacetamide (TAA)-induced hepatic encephalopathy (HE) in rats by injection of TAA (100 mg/kg, i.p) three times per week for six consecutive weeks. TAA-injected rats were administered ARG (100 mg/kg; p.o.) concurrently with TAA for the six consecutive weeks. Blood samples were withdrawn, and rats were sacrificed; liver and brain tissues were isolated. Results of the present study demonstrated that ARG administration to TAA-injected rats revealed a restoration in the serum and brain ammonia levels as well as serum aspartate transaminase, alanine transaminase, and alkaline phosphatase and total bilirubin levels as well as behavioral alterations evidenced by restoration in locomotor activity, motor skill performance, and memory impairment. ARG showed also improvement in the hepatic and neuro-biochemical values, pro-inflammatory cytokines, and oxidative stress biomarkers. All these results were confirmed by histopathological evaluation as well as ultrastructural imaging of the cerebellum using a transmission electron microscope. Furthermore, treatment with ARG could ameliorate the immunological reactivity of nuclear factor erythroid-2-related factor 2 (Nrf2) and cleaved caspase-3 proteins in the cerebellum and hepatic tissues. From all the previous results, it can be fulfilled that ARG showed a beneficial role in modulating the adverse complications associated with TAA-induced HE in rats via reducing hyperammonemia and downregulating nuclear factor kappa B (NF-κB)-mediated apoptosis.
ISSN:1614-7499
0944-1344
1614-7499
DOI:10.1007/s11356-023-28184-7