An integrative approach assesses the intraspecific variations of Procamallanus (Spirocamallanus) inopinatus, a common parasite in Neotropical freshwater fishes, and the phylogenetic patterns of Camallanidae

Integrative taxonomy was used to evaluate two component populations of Procamallanus (Spirocamallanus) inopinatus in Brazil and the phylogeny Camallanidae. Parasite populations were collected in the characiform Anostomoides passionis from River Xingu (Amazon basin) and Megaleporinus elongatus from R...

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Bibliographic Details
Published in:Parasitology 2020-12, Vol.147 (14), p.1752-1764
Main Authors: Ailán-Choke, Lorena G., Tavares, Luiz E.R., Luque, José L., Pereira, Felipe B.
Format: Article
Language:English
Subjects:
Animals
Brazil - epidemiology
Camallanidae
Characiformes
DNA, Helminth - analysis
DNA, Mitochondrial - analysis
Electron Transport Complex IV - analysis
Ethanol
Fish Diseases - epidemiology
Fish Diseases - parasitology
Freshwater fish
Generalized linear models
Host-Parasite Interactions
Infections
Labeling
Males
Microscopy - veterinary
Microscopy, Electron, Scanning - veterinary
Mitochondrial DNA
Morphology
Morphometry
Nematodes
Parasites
Phylogenetics
Phylogeny
Populations
Prevalence
Procamallanus
Regression analysis
River basins
Rivers
RNA, Helminth - analysis
RNA, Ribosomal, 18S - analysis
RNA, Ribosomal, 28S - analysis
Scanning electron microscopy
Spirocamallanus
Spirurida Infections - epidemiology
Spirurida Infections - parasitology
Spirurida Infections - veterinary
Spirurina - anatomy & histology
Spirurina - classification
Spirurina - genetics
Spirurina - physiology
Taxonomy
Uterus
Variables
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Summary:Integrative taxonomy was used to evaluate two component populations of Procamallanus (Spirocamallanus) inopinatus in Brazil and the phylogeny Camallanidae. Parasite populations were collected in the characiform Anostomoides passionis from River Xingu (Amazon basin) and Megaleporinus elongatus from River Miranda (Paraguay basin). Morphology was analysed using light and scanning electron microscopy (SEM). Genetic characterization was based on partial sequences of the 18S and 28S rDNA, and COI mtDNA. Phylogenies were based on 18S and COI due to data availability. Generalized Mixed Yule Coalescent (GMYC), Poisson Tree Process (PTP) and *BEAST were used for species delimitation and validation. SEM revealed for the first time the presence of minute denticles and pore-like structures surrounding the oral opening, phasmids in females and confirmed other important morphological aspects. Statistical comparison between the two-component populations indicated morphometric variations, especially among males. The different component population of P. (S.) inopinatus showed variable morphometry, but uniform morphology and were validated as conspecific by the GMYC, PTP and *BEAST. Some camallanid sequences in GenBank have incorrect taxonomic labelling. Host, environment and geographic aspects seem to be related to some lineages within Camallanidae; however, their real phylogenetic meanings are still unclear.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182020001687