Fluorescent indicators for imaging membrane potential of organelles

Plasma membrane potential is a key driver of the physiology of excitable cells like neurons and cardiomyocytes. Voltage-sensitive fluorescent indicators offer a powerful complement to traditional electrode-based approaches to measuring and monitoring membrane potential. Intracellular organelles can...

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Bibliographic Details
Published in:Current opinion in chemical biology 2022-12, Vol.71, p.102203-102203, Article 102203
Main Authors: Klier, Pavel E Z, Roo, Ryan, Miller, Evan W
Format: Article
Language:English
Subjects:
Diagnostic Imaging
Fluorescent Dyes - metabolism
Membrane Potentials
Neurons - physiology
Organelles - metabolism
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Summary:Plasma membrane potential is a key driver of the physiology of excitable cells like neurons and cardiomyocytes. Voltage-sensitive fluorescent indicators offer a powerful complement to traditional electrode-based approaches to measuring and monitoring membrane potential. Intracellular organelles can also generate membrane potential, yet the electrode- and fluorescent indicator-based approaches used for plasma membrane potential imaging are difficult to implement on intact organelles in their native environment. Here, we survey recent advances in developing and deploying voltage-sensitive fluorescent indicators to interrogate organelle membrane potential in intact cells.
ISSN:1367-5931
1879-0402
DOI:10.1016/j.cbpa.2022.102203