The Substitution of Fifty Percent of Combustible Tobacco Smoke Exposure With Either Electronic Cigarettes or Heated tobacco Products Did Not Attenuate Acute Lung Injury in an Animal Model

Abstract Background To reduce the harmful health effects of combustible cigarette smoke (CS), some (CS) users attempt to substitute CS with electronic cigarettes (ECIG) and/or heated tobacco products (HTP). In this animal study, we evaluated the acute effects of substituting CS consumption with ECIG...

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Bibliographic Details
Published in:Nicotine & tobacco research 2023-06, Vol.25 (7), p.1361-1368
Main Authors: Husari, Ahmad, El-Harakeh, Mohammad, Shihadeh, Alan, Daou, Michella Abi Zeid, Bitar, Hala, Karaoghlanian, Nareg, Zaatari, Ghazi, El-Sabban, Marwan
Format: Article
Language:English
Subjects:
Acute Lung Injury - chemically induced
Acute Lung Injury - therapy
Albumins
Animals
Disease Models, Animal
Electronic Nicotine Delivery Systems
Interleukin-6
Mice
Mice, Inbred C57BL
Original Investigations
Reactive Oxygen Species
Tobacco Products - adverse effects
Tobacco Smoke Pollution
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Summary:Abstract Background To reduce the harmful health effects of combustible cigarette smoke (CS), some (CS) users attempt to substitute CS with electronic cigarettes (ECIG) and/or heated tobacco products (HTP). In this animal study, we evaluated the acute effects of substituting CS consumption with ECIG or HTP thus mimicking the dual users’ approach, on the lungs of a mouse model. Methods C57BL/6 mice were divided into Control, ECIG, HTP, CS, ECIG + CS, HTP + CS, and HTP + ECIG groups. Animals were exposed for 3 hours in AM and PM sessions to either air, CS, ECIG, or HTP for seven days. Lung injury was assessed by: wet to dry (W/D) ratio, albumin concentration in bronchoalveolar lavage fluid, expression of IL-1β, IL-6, and TNF-α, histopathology examination, reactive oxygen species (ROS) production, and assessment of cellular apoptosis. Results W/D ratio was significantly increased in mice exposed to CS only. Albumin leak and expression of IL-1β, IL-6, and TNF-a were elevated in CS, ECIG + CS, and HTP + CS. Histological examination revealed significant inflammatory cells infiltration, as well as collagen deposit in CS, ECIG + CS, HTP + CS. ROS production was significantly increased in CS, ECIG + CS, HTP + CS. Finally, cell death was also significantly increased in CS, ECIG + CS, and HTP + CS. Conclusion In this animal model, substituting 50% of daily CS exposure by either ECIG or HTP exposure did not result in significant attenuation of acute lung injury.
ISSN:1469-994X
1462-2203
1469-994X
DOI:10.1093/ntr/ntad045