Microbial electrosynthesis with Clostridium ljungdahlii benefits from hydrogen electron mediation and permits a greater variety of products

Microbial electrosynthesis (MES) is a very promising technology addressing the challenge of carbon dioxide recycling into organic compounds, which might serve as building blocks for the (bio)chemical industry. However, poor process control and understanding of fundamental aspects such as the microbi...

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Bibliographic Details
Published in:Green chemistry : an international journal and green chemistry resource : GC 2023-06, Vol.25 (11), p.4375-4386
Main Authors: Boto, Santiago T, Bardl, Bettina, Harnisch, Falk, Rosenbaum, Miriam A
Format: Article
Language:English
Subjects:
Acetic acid
Biofilms
Biosynthesis
Carbon dioxide
Catalysts
Chemical industry
Chemistry
Clostridium ljungdahlii
Electron transfer
Electrophysiology
Ethanolamine
Glycine
Green chemistry
Hydrogen
Microorganisms
Organic compounds
Process control
Process controls
Process engineering
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Summary:Microbial electrosynthesis (MES) is a very promising technology addressing the challenge of carbon dioxide recycling into organic compounds, which might serve as building blocks for the (bio)chemical industry. However, poor process control and understanding of fundamental aspects such as the microbial extracellular electron transfer (EET) currently limit further developments. In the model acetogen , both direct and indirect electron consumption hydrogen have been proposed. However, without clarification neither targeted development of the microbial catalyst nor process engineering of MES are possible. In this study, cathodic hydrogen is demonstrated to be the dominating electron source for at electroautotrophic MES allowing for superior growth and biosynthesis, compared to previously reported MES using pure cultures. Hydrogen availability distinctly controlled an either planktonic- or biofilm-dominated lifestyle of . The most robust operation yielded higher planktonic cell densities in a hydrogen mediated process, which demonstrated the uncoupling of growth and biofilm formation. This coincided with an increase of metabolic activity, acetate titers, and production rates (up to 6.06 g L at 0.11 g L d ). For the first time, MES using was also revealed to deliver other products than acetate in significant amounts: here up to 0.39 g L glycine or 0.14 g L ethanolamine. Hence, a deeper comprehension of the electrophysiology of was shown to be key for designing and improving bioprocess strategies in MES research.
ISSN:1463-9262
1463-9270
1463-9262
DOI:10.1039/d3gc00471f