An RNA-based feed-forward mechanism ensures motor switching in oskar mRNA transport

Regulated recruitment and activity of motor proteins is essential for intracellular transport of cargoes, including messenger ribonucleoprotein complexes (RNPs). Here, we show that orchestration of oskar RNP transport in the Drosophila germline relies on interplay between two double-stranded RNA-bin...

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Bibliographic Details
Published in:The Journal of cell biology 2023-07, Vol.222 (7), p.1
Main Authors: Gáspár, Imre, Phea, Ly Jane, McClintock, Mark A, Heber, Simone, Bullock, Simon L, Ephrussi, Anne
Format: Article
Language:English
Subjects:
Animals
Biophysics
Cytoskeleton
Development
Double-stranded RNA
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Dynein
Dyneins - genetics
Dyneins - metabolism
Gametocytes
Kinesin
Kinesins - genetics
Kinesins - metabolism
Molecular motors
mRNA
Nurse cells
Oocytes - metabolism
Proteins
Ribonucleoproteins - metabolism
RNA biology
RNA Transport
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA-binding protein
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
Switching
Translocation
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Summary:Regulated recruitment and activity of motor proteins is essential for intracellular transport of cargoes, including messenger ribonucleoprotein complexes (RNPs). Here, we show that orchestration of oskar RNP transport in the Drosophila germline relies on interplay between two double-stranded RNA-binding proteins, Staufen and the dynein adaptor Egalitarian (Egl). We find that Staufen antagonizes Egl-mediated transport of oskar mRNA by dynein both in vitro and in vivo. Following delivery of nurse cell-synthesized oskar mRNA into the oocyte by dynein, recruitment of Staufen to the RNPs results in dissociation of Egl and a switch to kinesin-1-mediated translocation of the mRNA to its final destination at the posterior pole of the oocyte. We additionally show that Egl associates with staufen (stau) mRNA in the nurse cells, mediating its enrichment and translation in the ooplasm. Our observations identify a novel feed-forward mechanism, whereby dynein-dependent accumulation of stau mRNA, and thus protein, in the oocyte enables motor switching on oskar RNPs by downregulating dynein activity.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.202301113