SPICY: a method for single scan rotating frame relaxometry

T 1 ρ is an NMR relaxation mode that is sensitive to low frequency molecular motions, making it an especially valuable tool in biomolecular research. Here, we introduce a new method, SPICY, for measuring T 1 ρ relaxation times. In contrast to conventional T 1 ρ experiments, in which the sequence is...

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Bibliographic Details
Published in:Physical chemistry chemical physics : PCCP 2023-05, Vol.25 (18), p.13164-13169
Main Authors: Tolkkinen, Katja, Mailhiot, Sarah E, Selent, Anne, Mankinen, Otto, Henschel, Henning, Nieminen, Miika T, Hanni, Matti, Kantola, Anu M, Liimatainen, Timo, Telkki, Ville-Veikko
Format: Article
Language:English
Subjects:
Chemistry
NMR
Nuclear magnetic resonance
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Summary:T 1 ρ is an NMR relaxation mode that is sensitive to low frequency molecular motions, making it an especially valuable tool in biomolecular research. Here, we introduce a new method, SPICY, for measuring T 1 ρ relaxation times. In contrast to conventional T 1 ρ experiments, in which the sequence is repeated many times to determine the T 1 ρ time, the SPICY sequence allows determination of T 1 ρ within a single scan, shortening the experiment time remarkably. We demonstrate the method using 1 H T 1 ρ relaxation dispersion experiments. Additionally, we combine the sequence with spatial encoding to produce 1D images in a single scan. We show that T 1 ρ relaxation times obtained using the single scan approach are in good agreement with those obtained using the traditional experiments. The SPICY NMR method enables the determination of T 1 ρ relaxation times and 1D T 1 ρ imaging in a single scan to elucidate low frequency molecular motions.
ISSN:1463-9076
1463-9084
1463-9084
DOI:10.1039/d2cp05988f