Loading…
Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig
One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that ar...
Saved in:
Published in: | Cell transplantation 2023-01, Vol.32, p.9636897221107009-9636897221107009 |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | |
---|---|
cites | cdi_FETCH-LOGICAL-c419t-c451527221ad2d2938a8a2f701b8e21a018eec3e43f74131dd78eac97a4947543 |
container_end_page | 9636897221107009 |
container_issue | |
container_start_page | 9636897221107009 |
container_title | Cell transplantation |
container_volume | 32 |
creator | Kobayashi, Yoshiomi Shigyo, Michiko Platoshyn, Oleksandr Marsala, Silvia Kato, Tomohisa Takamura, Naoki Yoshida, Kenji Kishino, Akiyoshi Bravo-Hernandez, Mariana Juhas, Stefan Juhasova, Jana Studenovska, Hana Proks, Vladimir Driscoll, Shawn P. Glenn, Thomas D. Pfaff, Samuel L. Ciacci, Joseph D. Marsala, Martin |
description | One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that are simple to use is critical for potential future dissemination of any spinally targeted cell-replacement therapy into general medical practice. Here, we compared the engraftment properties of established human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cell (NPCs) line once cells were harvested fresh from the cell culture or previously frozen and then grafted into striata or spinal cord of the immunodeficient rat. A newly developed human spinal injection device equipped with a spinal cord pulsation-cancelation magnetic needle was also tested for its safety in an adult immunosuppressed pig. Previously frozen NPCs showed similar post-grafting survival and differentiation profile as was seen for freshly harvested cells. Testing of human injection device showed acceptable safety with no detectable surgical procedure or spinal NPCs injection-related side effects. |
doi_str_mv | 10.1177/09636897221107009 |
format | article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10134149</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_09636897221107009</sage_id><sourcerecordid>3095716692</sourcerecordid><originalsourceid>FETCH-LOGICAL-c419t-c451527221ad2d2938a8a2f701b8e21a018eec3e43f74131dd78eac97a4947543</originalsourceid><addsrcrecordid>eNp1kc1u1DAUhS0EokPhAdggS2zYpNhxHNtsUDUqbaUKRh3o1rqT3KSuMvHUdiraJ-hj49GU8ic2tuX73XN9fAh5zdkB50q9Z6YWtTaqLDlnijHzhMy4lLIQ2pRPyWxbL7bAHnkR4xVjTIlSPid7QjGtjVYzcn_0fQNjC6sB6RLzwRUXLkyxOMdN8H2A9RpbejKtYaRusZwXn3EKfoSBLgI2U4g-xA_0dKQX7sbThY-pOA7QJTf2dAkdpls6v4QATcLg7iA5n3VGeg4p0jyXHrbTkOjC9S_Jsw6GiK8e9n3y7dPR1_lJcfbl-HR-eFY0FTcpr5LLcusY2rItjdCgoewU4yuN-ZJxjdgIrESnKi542yqN0BgFlamUrMQ--bjT3UyrbK3BMQUY7Ca4NYRb68HZPyuju7S9v7GccVHxymSFdw8KwV9PGJNdu9jgMMCIfoq21ExKbmrJMvr2L_TKTyH_XrSCGal4XZsyU3xHNcHHGLB7fA1ndhu0_Sfo3PPmdxuPHT-TzcDBDojQ46-x_1f8AUk5sUo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3095716692</pqid></control><display><type>article</type><title>Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig</title><source>SAGE Open Access Journals</source><source>ProQuest - Publicly Available Content Database</source><source>PubMed Central</source><creator>Kobayashi, Yoshiomi ; Shigyo, Michiko ; Platoshyn, Oleksandr ; Marsala, Silvia ; Kato, Tomohisa ; Takamura, Naoki ; Yoshida, Kenji ; Kishino, Akiyoshi ; Bravo-Hernandez, Mariana ; Juhas, Stefan ; Juhasova, Jana ; Studenovska, Hana ; Proks, Vladimir ; Driscoll, Shawn P. ; Glenn, Thomas D. ; Pfaff, Samuel L. ; Ciacci, Joseph D. ; Marsala, Martin</creator><creatorcontrib>Kobayashi, Yoshiomi ; Shigyo, Michiko ; Platoshyn, Oleksandr ; Marsala, Silvia ; Kato, Tomohisa ; Takamura, Naoki ; Yoshida, Kenji ; Kishino, Akiyoshi ; Bravo-Hernandez, Mariana ; Juhas, Stefan ; Juhasova, Jana ; Studenovska, Hana ; Proks, Vladimir ; Driscoll, Shawn P. ; Glenn, Thomas D. ; Pfaff, Samuel L. ; Ciacci, Joseph D. ; Marsala, Martin</creatorcontrib><description>One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that are simple to use is critical for potential future dissemination of any spinally targeted cell-replacement therapy into general medical practice. Here, we compared the engraftment properties of established human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cell (NPCs) line once cells were harvested fresh from the cell culture or previously frozen and then grafted into striata or spinal cord of the immunodeficient rat. A newly developed human spinal injection device equipped with a spinal cord pulsation-cancelation magnetic needle was also tested for its safety in an adult immunosuppressed pig. Previously frozen NPCs showed similar post-grafting survival and differentiation profile as was seen for freshly harvested cells. Testing of human injection device showed acceptable safety with no detectable surgical procedure or spinal NPCs injection-related side effects.</description><identifier>ISSN: 0963-6897</identifier><identifier>EISSN: 1555-3892</identifier><identifier>DOI: 10.1177/09636897221107009</identifier><identifier>PMID: 37088987</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Adult ; Animals ; Brain ; Cell culture ; Cell differentiation ; Cell Differentiation - physiology ; Cell survival ; Cellular Reprogramming - genetics ; Cellular Reprogramming - physiology ; Genetic Vectors - genetics ; Graft Survival - physiology ; Humans ; Immunodeficiency ; Induced Pluripotent Stem Cells - physiology ; Induced Pluripotent Stem Cells - transplantation ; Injection ; Injections, Spinal - adverse effects ; Injections, Spinal - instrumentation ; Injections, Spinal - methods ; Neural stem cells ; Neural Stem Cells - physiology ; Neural Stem Cells - transplantation ; Original ; Pluripotency ; Rats ; Sendai virus ; Specimen Handling - methods ; Spinal Cord ; Stem Cell Transplantation - adverse effects ; Stem Cell Transplantation - instrumentation ; Stem Cell Transplantation - methods ; Swine ; Tissue and Organ Harvesting - methods ; Treatment Outcome</subject><ispartof>Cell transplantation, 2023-01, Vol.32, p.9636897221107009-9636897221107009</ispartof><rights>The Author(s) 2023</rights><rights>The Author(s) 2023. This work is licensed under the Creative Commons Attribution License https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2023 2023 SAGE Publications Inc, unless otherwise noted. Manuscript content on this site is licensed under Creative Commons Licenses</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c419t-c451527221ad2d2938a8a2f701b8e21a018eec3e43f74131dd78eac97a4947543</cites><orcidid>0000-0001-5048-6422</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134149/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3095716692?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,21994,25783,27886,27957,27958,37047,37048,44625,44980,45368,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37088987$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kobayashi, Yoshiomi</creatorcontrib><creatorcontrib>Shigyo, Michiko</creatorcontrib><creatorcontrib>Platoshyn, Oleksandr</creatorcontrib><creatorcontrib>Marsala, Silvia</creatorcontrib><creatorcontrib>Kato, Tomohisa</creatorcontrib><creatorcontrib>Takamura, Naoki</creatorcontrib><creatorcontrib>Yoshida, Kenji</creatorcontrib><creatorcontrib>Kishino, Akiyoshi</creatorcontrib><creatorcontrib>Bravo-Hernandez, Mariana</creatorcontrib><creatorcontrib>Juhas, Stefan</creatorcontrib><creatorcontrib>Juhasova, Jana</creatorcontrib><creatorcontrib>Studenovska, Hana</creatorcontrib><creatorcontrib>Proks, Vladimir</creatorcontrib><creatorcontrib>Driscoll, Shawn P.</creatorcontrib><creatorcontrib>Glenn, Thomas D.</creatorcontrib><creatorcontrib>Pfaff, Samuel L.</creatorcontrib><creatorcontrib>Ciacci, Joseph D.</creatorcontrib><creatorcontrib>Marsala, Martin</creatorcontrib><title>Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig</title><title>Cell transplantation</title><addtitle>Cell Transplant</addtitle><description>One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that are simple to use is critical for potential future dissemination of any spinally targeted cell-replacement therapy into general medical practice. Here, we compared the engraftment properties of established human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cell (NPCs) line once cells were harvested fresh from the cell culture or previously frozen and then grafted into striata or spinal cord of the immunodeficient rat. A newly developed human spinal injection device equipped with a spinal cord pulsation-cancelation magnetic needle was also tested for its safety in an adult immunosuppressed pig. Previously frozen NPCs showed similar post-grafting survival and differentiation profile as was seen for freshly harvested cells. Testing of human injection device showed acceptable safety with no detectable surgical procedure or spinal NPCs injection-related side effects.</description><subject>Adult</subject><subject>Animals</subject><subject>Brain</subject><subject>Cell culture</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - physiology</subject><subject>Cell survival</subject><subject>Cellular Reprogramming - genetics</subject><subject>Cellular Reprogramming - physiology</subject><subject>Genetic Vectors - genetics</subject><subject>Graft Survival - physiology</subject><subject>Humans</subject><subject>Immunodeficiency</subject><subject>Induced Pluripotent Stem Cells - physiology</subject><subject>Induced Pluripotent Stem Cells - transplantation</subject><subject>Injection</subject><subject>Injections, Spinal - adverse effects</subject><subject>Injections, Spinal - instrumentation</subject><subject>Injections, Spinal - methods</subject><subject>Neural stem cells</subject><subject>Neural Stem Cells - physiology</subject><subject>Neural Stem Cells - transplantation</subject><subject>Original</subject><subject>Pluripotency</subject><subject>Rats</subject><subject>Sendai virus</subject><subject>Specimen Handling - methods</subject><subject>Spinal Cord</subject><subject>Stem Cell Transplantation - adverse effects</subject><subject>Stem Cell Transplantation - instrumentation</subject><subject>Stem Cell Transplantation - methods</subject><subject>Swine</subject><subject>Tissue and Organ Harvesting - methods</subject><subject>Treatment Outcome</subject><issn>0963-6897</issn><issn>1555-3892</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>AFRWT</sourceid><sourceid>PIMPY</sourceid><recordid>eNp1kc1u1DAUhS0EokPhAdggS2zYpNhxHNtsUDUqbaUKRh3o1rqT3KSuMvHUdiraJ-hj49GU8ic2tuX73XN9fAh5zdkB50q9Z6YWtTaqLDlnijHzhMy4lLIQ2pRPyWxbL7bAHnkR4xVjTIlSPid7QjGtjVYzcn_0fQNjC6sB6RLzwRUXLkyxOMdN8H2A9RpbejKtYaRusZwXn3EKfoSBLgI2U4g-xA_0dKQX7sbThY-pOA7QJTf2dAkdpls6v4QATcLg7iA5n3VGeg4p0jyXHrbTkOjC9S_Jsw6GiK8e9n3y7dPR1_lJcfbl-HR-eFY0FTcpr5LLcusY2rItjdCgoewU4yuN-ZJxjdgIrESnKi542yqN0BgFlamUrMQ--bjT3UyrbK3BMQUY7Ca4NYRb68HZPyuju7S9v7GccVHxymSFdw8KwV9PGJNdu9jgMMCIfoq21ExKbmrJMvr2L_TKTyH_XrSCGal4XZsyU3xHNcHHGLB7fA1ndhu0_Sfo3PPmdxuPHT-TzcDBDojQ46-x_1f8AUk5sUo</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Kobayashi, Yoshiomi</creator><creator>Shigyo, Michiko</creator><creator>Platoshyn, Oleksandr</creator><creator>Marsala, Silvia</creator><creator>Kato, Tomohisa</creator><creator>Takamura, Naoki</creator><creator>Yoshida, Kenji</creator><creator>Kishino, Akiyoshi</creator><creator>Bravo-Hernandez, Mariana</creator><creator>Juhas, Stefan</creator><creator>Juhasova, Jana</creator><creator>Studenovska, Hana</creator><creator>Proks, Vladimir</creator><creator>Driscoll, Shawn P.</creator><creator>Glenn, Thomas D.</creator><creator>Pfaff, Samuel L.</creator><creator>Ciacci, Joseph D.</creator><creator>Marsala, Martin</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>AFRWT</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5048-6422</orcidid></search><sort><creationdate>20230101</creationdate><title>Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig</title><author>Kobayashi, Yoshiomi ; Shigyo, Michiko ; Platoshyn, Oleksandr ; Marsala, Silvia ; Kato, Tomohisa ; Takamura, Naoki ; Yoshida, Kenji ; Kishino, Akiyoshi ; Bravo-Hernandez, Mariana ; Juhas, Stefan ; Juhasova, Jana ; Studenovska, Hana ; Proks, Vladimir ; Driscoll, Shawn P. ; Glenn, Thomas D. ; Pfaff, Samuel L. ; Ciacci, Joseph D. ; Marsala, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-c451527221ad2d2938a8a2f701b8e21a018eec3e43f74131dd78eac97a4947543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Brain</topic><topic>Cell culture</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - physiology</topic><topic>Cell survival</topic><topic>Cellular Reprogramming - genetics</topic><topic>Cellular Reprogramming - physiology</topic><topic>Genetic Vectors - genetics</topic><topic>Graft Survival - physiology</topic><topic>Humans</topic><topic>Immunodeficiency</topic><topic>Induced Pluripotent Stem Cells - physiology</topic><topic>Induced Pluripotent Stem Cells - transplantation</topic><topic>Injection</topic><topic>Injections, Spinal - adverse effects</topic><topic>Injections, Spinal - instrumentation</topic><topic>Injections, Spinal - methods</topic><topic>Neural stem cells</topic><topic>Neural Stem Cells - physiology</topic><topic>Neural Stem Cells - transplantation</topic><topic>Original</topic><topic>Pluripotency</topic><topic>Rats</topic><topic>Sendai virus</topic><topic>Specimen Handling - methods</topic><topic>Spinal Cord</topic><topic>Stem Cell Transplantation - adverse effects</topic><topic>Stem Cell Transplantation - instrumentation</topic><topic>Stem Cell Transplantation - methods</topic><topic>Swine</topic><topic>Tissue and Organ Harvesting - methods</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kobayashi, Yoshiomi</creatorcontrib><creatorcontrib>Shigyo, Michiko</creatorcontrib><creatorcontrib>Platoshyn, Oleksandr</creatorcontrib><creatorcontrib>Marsala, Silvia</creatorcontrib><creatorcontrib>Kato, Tomohisa</creatorcontrib><creatorcontrib>Takamura, Naoki</creatorcontrib><creatorcontrib>Yoshida, Kenji</creatorcontrib><creatorcontrib>Kishino, Akiyoshi</creatorcontrib><creatorcontrib>Bravo-Hernandez, Mariana</creatorcontrib><creatorcontrib>Juhas, Stefan</creatorcontrib><creatorcontrib>Juhasova, Jana</creatorcontrib><creatorcontrib>Studenovska, Hana</creatorcontrib><creatorcontrib>Proks, Vladimir</creatorcontrib><creatorcontrib>Driscoll, Shawn P.</creatorcontrib><creatorcontrib>Glenn, Thomas D.</creatorcontrib><creatorcontrib>Pfaff, Samuel L.</creatorcontrib><creatorcontrib>Ciacci, Joseph D.</creatorcontrib><creatorcontrib>Marsala, Martin</creatorcontrib><collection>SAGE Open Access Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest - Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kobayashi, Yoshiomi</au><au>Shigyo, Michiko</au><au>Platoshyn, Oleksandr</au><au>Marsala, Silvia</au><au>Kato, Tomohisa</au><au>Takamura, Naoki</au><au>Yoshida, Kenji</au><au>Kishino, Akiyoshi</au><au>Bravo-Hernandez, Mariana</au><au>Juhas, Stefan</au><au>Juhasova, Jana</au><au>Studenovska, Hana</au><au>Proks, Vladimir</au><au>Driscoll, Shawn P.</au><au>Glenn, Thomas D.</au><au>Pfaff, Samuel L.</au><au>Ciacci, Joseph D.</au><au>Marsala, Martin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig</atitle><jtitle>Cell transplantation</jtitle><addtitle>Cell Transplant</addtitle><date>2023-01-01</date><risdate>2023</risdate><volume>32</volume><spage>9636897221107009</spage><epage>9636897221107009</epage><pages>9636897221107009-9636897221107009</pages><issn>0963-6897</issn><eissn>1555-3892</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Yoshiomi Kobayashi is also affilated to The National Medical Center for Spine and Spinal Cord disease, Murayama Medical Center, Gakuen, Musashimurayama city, Tokyo, Japan</notes><notes>Co-first author</notes><abstract>One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that are simple to use is critical for potential future dissemination of any spinally targeted cell-replacement therapy into general medical practice. Here, we compared the engraftment properties of established human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cell (NPCs) line once cells were harvested fresh from the cell culture or previously frozen and then grafted into striata or spinal cord of the immunodeficient rat. A newly developed human spinal injection device equipped with a spinal cord pulsation-cancelation magnetic needle was also tested for its safety in an adult immunosuppressed pig. Previously frozen NPCs showed similar post-grafting survival and differentiation profile as was seen for freshly harvested cells. Testing of human injection device showed acceptable safety with no detectable surgical procedure or spinal NPCs injection-related side effects.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>37088987</pmid><doi>10.1177/09636897221107009</doi><orcidid>https://orcid.org/0000-0001-5048-6422</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0963-6897 |
ispartof | Cell transplantation, 2023-01, Vol.32, p.9636897221107009-9636897221107009 |
issn | 0963-6897 1555-3892 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10134149 |
source | SAGE Open Access Journals; ProQuest - Publicly Available Content Database; PubMed Central |
subjects | Adult Animals Brain Cell culture Cell differentiation Cell Differentiation - physiology Cell survival Cellular Reprogramming - genetics Cellular Reprogramming - physiology Genetic Vectors - genetics Graft Survival - physiology Humans Immunodeficiency Induced Pluripotent Stem Cells - physiology Induced Pluripotent Stem Cells - transplantation Injection Injections, Spinal - adverse effects Injections, Spinal - instrumentation Injections, Spinal - methods Neural stem cells Neural Stem Cells - physiology Neural Stem Cells - transplantation Original Pluripotency Rats Sendai virus Specimen Handling - methods Spinal Cord Stem Cell Transplantation - adverse effects Stem Cell Transplantation - instrumentation Stem Cell Transplantation - methods Swine Tissue and Organ Harvesting - methods Treatment Outcome |
title | Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T08%3A54%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expandable%20Sendai-Virus-Reprogrammed%20Human%20iPSC-Neuronal%20Precursors:%20In%20Vivo%20Post-Grafting%20Safety%20Characterization%20in%20Rats%20and%20Adult%20Pig&rft.jtitle=Cell%20transplantation&rft.au=Kobayashi,%20Yoshiomi&rft.date=2023-01-01&rft.volume=32&rft.spage=9636897221107009&rft.epage=9636897221107009&rft.pages=9636897221107009-9636897221107009&rft.issn=0963-6897&rft.eissn=1555-3892&rft_id=info:doi/10.1177/09636897221107009&rft_dat=%3Cproquest_pubme%3E3095716692%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c419t-c451527221ad2d2938a8a2f701b8e21a018eec3e43f74131dd78eac97a4947543%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3095716692&rft_id=info:pmid/37088987&rft_sage_id=10.1177_09636897221107009&rfr_iscdi=true |