Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig

One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that ar...

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Bibliographic Details
Published in:Cell transplantation 2023-01, Vol.32, p.9636897221107009-9636897221107009
Main Authors: Kobayashi, Yoshiomi, Shigyo, Michiko, Platoshyn, Oleksandr, Marsala, Silvia, Kato, Tomohisa, Takamura, Naoki, Yoshida, Kenji, Kishino, Akiyoshi, Bravo-Hernandez, Mariana, Juhas, Stefan, Juhasova, Jana, Studenovska, Hana, Proks, Vladimir, Driscoll, Shawn P., Glenn, Thomas D., Pfaff, Samuel L., Ciacci, Joseph D., Marsala, Martin
Format: Article
Language:English
Subjects:
Adult
Animals
Brain
Cell culture
Cell differentiation
Cell Differentiation - physiology
Cell survival
Cellular Reprogramming - genetics
Cellular Reprogramming - physiology
Genetic Vectors - genetics
Graft Survival - physiology
Humans
Immunodeficiency
Induced Pluripotent Stem Cells - physiology
Induced Pluripotent Stem Cells - transplantation
Injection
Injections, Spinal - adverse effects
Injections, Spinal - instrumentation
Injections, Spinal - methods
Neural stem cells
Neural Stem Cells - physiology
Neural Stem Cells - transplantation
Original
Pluripotency
Rats
Sendai virus
Specimen Handling - methods
Spinal Cord
Stem Cell Transplantation - adverse effects
Stem Cell Transplantation - instrumentation
Stem Cell Transplantation - methods
Swine
Tissue and Organ Harvesting - methods
Treatment Outcome
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Summary:One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that are simple to use is critical for potential future dissemination of any spinally targeted cell-replacement therapy into general medical practice. Here, we compared the engraftment properties of established human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cell (NPCs) line once cells were harvested fresh from the cell culture or previously frozen and then grafted into striata or spinal cord of the immunodeficient rat. A newly developed human spinal injection device equipped with a spinal cord pulsation-cancelation magnetic needle was also tested for its safety in an adult immunosuppressed pig. Previously frozen NPCs showed similar post-grafting survival and differentiation profile as was seen for freshly harvested cells. Testing of human injection device showed acceptable safety with no detectable surgical procedure or spinal NPCs injection-related side effects.
ISSN:0963-6897
1555-3892
DOI:10.1177/09636897221107009