Type III secretion-dependent cell cycle block caused in HeLa cells by enteropathogenic Escherichia coli O103

Rabbit enteropathogenic Escherichia coli (EPEC) O103 induces in HeLa cells an irreversible cytopathic effect characterized by the recruitment of focal adhesions, formation of stress fibers, and inhibition of cell proliferation. We have characterized the modalities of the proliferation arrest and inv...

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Bibliographic Details
Published in:Infection and immunity 2001-11, Vol.69 (11), p.6785-6795
Main Authors: NOUGAYREDE, Jean-Philippe, BOURY, Michèle, TASCA, Christian, MARCHES, Olivier, MILON, Alain, OSWALD, Eric, DE RYCKE, Jean
Format: Article
Language:English
Subjects:
Adhesins, Bacterial
Bacterial Outer Membrane Proteins - metabolism
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
Carrier Proteins
CDC2 Protein Kinase - metabolism
Cell Cycle
Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
Cyclin B - metabolism
Cyclin B1
cyclin-dependent kinase 1
Cytoskeleton - physiology
Escherichia coli
Escherichia coli - metabolism
Escherichia coli - pathogenicity
Escherichia coli Proteins
Fundamental and applied biological sciences. Psychology
G2 Phase
Genetics
HeLa Cells
Humans
Life Sciences
Microbiology
Mitosis
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Phosphorylation
Receptors, Cell Surface - metabolism
Tyrosine - metabolism
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Summary:Rabbit enteropathogenic Escherichia coli (EPEC) O103 induces in HeLa cells an irreversible cytopathic effect characterized by the recruitment of focal adhesions, formation of stress fibers, and inhibition of cell proliferation. We have characterized the modalities of the proliferation arrest and investigated its underlying mechanisms. We found that HeLa cells that were exposed to the rabbit EPEC O103 strain E22 progressively accumulated at 4C DNA content and did not enter mitosis. A significant proportion of the cells were able to reinitiate DNA synthesis without division, leading to 8C DNA content. This cell cycle inhibition by E22 was abrogated in mutants lacking EspA, -B, and -D and was restored by transcomplementation. In contrast, intimin and Tir mutants retained the antiproliferative effect. The cell cycle arrest was not a direct consequence of the formation of stress fibers, since their disruption by toxins during exposure to E22 did not reverse the cell cycle inhibition. Likewise, the cell cycle arrest was not dependent on the early tyrosine dephosphorylation events triggered by E22 in the cells. Two key partner effectors controlling entry into mitosis were also investigated: cyclin B1 and the associated cyclin-dependent kinase 1 (Cdk1). Whereas cyclin B1 was not detectably affected in E22-exposed cells, Cdk1 was maintained in a tyrosine-phosphorylated inactive state and lost its affinity for p13(suc1)-agarose beads. This shows that Cdk1 is implicated in the G2/M arrest caused by EPEC strain E22.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.69.11.6785-6795.2001