Involvement of different transduction pathways in NF-kappa B activation by several inducers

Double-stimulation was used to demonstrate that, in a T lymphocytic cell line (CEM), phorbol myristate acetate (PMA) rapidly induced NF-kappa B through a signaling pathway which did not involve reactive oxygen species (ROS) and was different from the activation triggered by either H2O2 or tumor necr...

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Bibliographic Details
Published in:Free radical research 1997-09, Vol.27 (3), p.301
Main Authors: Legrand-Poels, S, Zecchinon, L, Piret, B, Schoonbroodt, S, Piette, J
Format: Article
Language:English
Subjects:
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine - pharmacology
Acetylcysteine - pharmacology
Antioxidants - pharmacology
Base Sequence
Binding Sites
Cell Line
Enzyme Inhibitors - pharmacology
Humans
Hydrogen Peroxide - pharmacology
Kinetics
Molecular Sequence Data
NF-kappa B - metabolism
Oligodeoxyribonucleotides - chemistry
Oligodeoxyribonucleotides - metabolism
Protein Kinase Inhibitors
Reactive Oxygen Species
Signal Transduction - drug effects
Staurosporine - pharmacology
T-Lymphocytes
Tetradecanoylphorbol Acetate - pharmacology
Tumor Necrosis Factor-alpha - pharmacology
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Summary:Double-stimulation was used to demonstrate that, in a T lymphocytic cell line (CEM), phorbol myristate acetate (PMA) rapidly induced NF-kappa B through a signaling pathway which did not involve reactive oxygen species (ROS) and was different from the activation triggered by either H2O2 or tumor necrosis factor-alpha (TNF-alpha). Since these latter compounds were known to activate NF-kappa B translocation in a redox-sensitive way, we have demonstrated that NF-kappa B activation by PMA was resistant to antioxidant N-acetyl-L-cysteine (NAC) and sensitive to kinase inhibitors staurosporine and H7 while activation by H2O2 or TNF-alpha were not.
ISSN:1071-5762
1029-2470
DOI:10.3109/10715769709065768