Suppression of L-histidine decarboxylase mRNA expression by methyleugenol

We investigated the effect of methyleugenol on anaphylaxis. Methyleugenol completely inhibited systemic anaphylaxis induced by compound 48/80 in mice. Methyleugenol also inhibited local anaphylaxis activated by anti-dinitrophenyl (DNP) IgE . More over, methyleugenol dose-dependently inhibited histam...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1997-03, Vol.232 (1), p.188-191
Main Authors: Shin, B.K, Lee, E.H, Kim, H.M
Format: Article
Language:English
Subjects:
anaphylaxis
Animals
Blotting, Northern
Eugenol - analogs & derivatives
Eugenol - pharmacology
Gene Expression Regulation - drug effects
Histidine Decarboxylase - genetics
Mast Cells - enzymology
messenger RNA
Mice
Mice, Inbred BALB C
Rats
Rats, Wistar
RNA, Messenger - genetics
Substance P - pharmacology
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Summary:We investigated the effect of methyleugenol on anaphylaxis. Methyleugenol completely inhibited systemic anaphylaxis induced by compound 48/80 in mice. Methyleugenol also inhibited local anaphylaxis activated by anti-dinitrophenyl (DNP) IgE . More over, methyleugenol dose-dependently inhibited histamine release in mast cells activated by compound 48/80 or anti-DNP IgE. Northern-blot analysis demonstrated that significantly reduced level of the mRNA of L-histidine decarboxylase (HDC) was expressed in mast cells treated with methyleugenol, compared to that without methyleugenol. We conclude that methyleugenol directly affect histamine release and HDC gene over-expression in mast cells.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1997.6260