Serologic markers in early stages of African horse sickness virus infection

Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to deve...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Clinical Microbiology 1997-02, Vol.35 (2), p.531-535
Main Authors: Martinez-Torrecuadrada, J.L. (INGENASA, Madrid.), Diaz-Laviada, M, Roy, P, Sanchez, C, Vela, C, Sanchez-Vizcaino, J.M, Casal, J.I
Format: Article
Language:English
Subjects:
African Horse Sickness - immunology
African horse sickness virus
African Horse Sickness Virus - immunology
Animals
Antibodies, Viral - blood
Antigens, Viral
Biological and medical sciences
Blotting, Western
Capsid - immunology
Capsid Proteins
Experimental viral diseases and models
INFECCION
INFECTION
Infectious diseases
Medical sciences
Vaccination
Viral diseases
Viral Proteins - immunology
Viral Vaccines - immunology
VIRUS DE LA PESTE EQUINA AFRICANA
VIRUS PESTE EQUINE AFRICAINE
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c561t-4ab7dd91d94af8b32a36115e6b5ad1086679bf762b0f55aa1de8177422e6ca053
cites
container_end_page 535
container_issue 2
container_start_page 531
container_title Journal of Clinical Microbiology
container_volume 35
creator Martinez-Torrecuadrada, J.L. (INGENASA, Madrid.)
Diaz-Laviada, M
Roy, P
Sanchez, C
Vela, C
Sanchez-Vizcaino, J.M
Casal, J.I
description Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to develop a humoral immune response detectable by immunoblotting. The earliest serological markers corresponded mainly to VP5, VP6, and NS2 and to a lesser extent to VP3, NS1, and NS3. Neutralizing antibodies to VP2 were not detected by immunoblotting, suggesting that they are mostly conformation dependent. VP7-specific antibodies were detected later in infection. These results make NS2 and VP6 the most attractive candidates for the rapid diagnosis of the infection
doi_str_mv 10.1128/jcm.35.2.531-535.1997
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_9003637</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78801896</sourcerecordid><originalsourceid>FETCH-LOGICAL-c561t-4ab7dd91d94af8b32a36115e6b5ad1086679bf762b0f55aa1de8177422e6ca053</originalsourceid><addsrcrecordid>eNqFkU1rFTEUhoMo9Vr9A0IhC3E313xMvhYuStEqFlzUgrtwJpPMTTszqcncSv99c7mXi125OOTA-7wn5_AidEbJmlKmP926ac3Fmq0Fp42oHTVGvUArSoxupCS_X6IVIUY0lHL1Gr0p5ZYQ2rZCnKATQwiXXK3Qj2uf05iG6PAE-c7nguOMPeTxEZcFBl9wCvg85OhgxpuUi8clurvZl4IfYt7u-ODdEtP8Fr0KMBb_7vCeopuvX35dfGuufl5-vzi_apyQdGla6FTfG9qbFoLuOAMuKRVedgJ6SrSUynRBSdaRIAQA7b2mSrWMeemACH6KPu_n3m-7yffOz0uG0d7nWE94tAmifa7McWOH9GAZM5LR6v948Of0Z-vLYqdYnB9HmH3aFqu0JlQb-V-QCl131ayCYg-6nErJPhyXocTu0rI1LcuFZbamVUvYXVrVd_bvJUfXIZ6qfzjoUByMIcPsYjliTLStYaRieI9t4rD5G7O3UKZnX1bk_R4JkCwMuU65uTaq5a0S_AkNK7KS</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15867982</pqid></control><display><type>article</type><title>Serologic markers in early stages of African horse sickness virus infection</title><source>Open Access: PubMed Central</source><source>American Society for Microbiology Journals</source><creator>Martinez-Torrecuadrada, J.L. (INGENASA, Madrid.) ; Diaz-Laviada, M ; Roy, P ; Sanchez, C ; Vela, C ; Sanchez-Vizcaino, J.M ; Casal, J.I</creator><creatorcontrib>Martinez-Torrecuadrada, J.L. (INGENASA, Madrid.) ; Diaz-Laviada, M ; Roy, P ; Sanchez, C ; Vela, C ; Sanchez-Vizcaino, J.M ; Casal, J.I</creatorcontrib><description>Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to develop a humoral immune response detectable by immunoblotting. The earliest serological markers corresponded mainly to VP5, VP6, and NS2 and to a lesser extent to VP3, NS1, and NS3. Neutralizing antibodies to VP2 were not detected by immunoblotting, suggesting that they are mostly conformation dependent. VP7-specific antibodies were detected later in infection. These results make NS2 and VP6 the most attractive candidates for the rapid diagnosis of the infection</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/jcm.35.2.531-535.1997</identifier><identifier>PMID: 9003637</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>African Horse Sickness - immunology ; African horse sickness virus ; African Horse Sickness Virus - immunology ; Animals ; Antibodies, Viral - blood ; Antigens, Viral ; Biological and medical sciences ; Blotting, Western ; Capsid - immunology ; Capsid Proteins ; Experimental viral diseases and models ; INFECCION ; INFECTION ; Infectious diseases ; Medical sciences ; Vaccination ; Viral diseases ; Viral Proteins - immunology ; Viral Vaccines - immunology ; VIRUS DE LA PESTE EQUINA AFRICANA ; VIRUS PESTE EQUINE AFRICAINE</subject><ispartof>Journal of Clinical Microbiology, 1997-02, Vol.35 (2), p.531-535</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c561t-4ab7dd91d94af8b32a36115e6b5ad1086679bf762b0f55aa1de8177422e6ca053</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC229621/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC229621/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,3207,3208,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=2544920$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9003637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martinez-Torrecuadrada, J.L. (INGENASA, Madrid.)</creatorcontrib><creatorcontrib>Diaz-Laviada, M</creatorcontrib><creatorcontrib>Roy, P</creatorcontrib><creatorcontrib>Sanchez, C</creatorcontrib><creatorcontrib>Vela, C</creatorcontrib><creatorcontrib>Sanchez-Vizcaino, J.M</creatorcontrib><creatorcontrib>Casal, J.I</creatorcontrib><title>Serologic markers in early stages of African horse sickness virus infection</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to develop a humoral immune response detectable by immunoblotting. The earliest serological markers corresponded mainly to VP5, VP6, and NS2 and to a lesser extent to VP3, NS1, and NS3. Neutralizing antibodies to VP2 were not detected by immunoblotting, suggesting that they are mostly conformation dependent. VP7-specific antibodies were detected later in infection. These results make NS2 and VP6 the most attractive candidates for the rapid diagnosis of the infection</description><subject>African Horse Sickness - immunology</subject><subject>African horse sickness virus</subject><subject>African Horse Sickness Virus - immunology</subject><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Antigens, Viral</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Capsid - immunology</subject><subject>Capsid Proteins</subject><subject>Experimental viral diseases and models</subject><subject>INFECCION</subject><subject>INFECTION</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Vaccination</subject><subject>Viral diseases</subject><subject>Viral Proteins - immunology</subject><subject>Viral Vaccines - immunology</subject><subject>VIRUS DE LA PESTE EQUINA AFRICANA</subject><subject>VIRUS PESTE EQUINE AFRICAINE</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkU1rFTEUhoMo9Vr9A0IhC3E313xMvhYuStEqFlzUgrtwJpPMTTszqcncSv99c7mXi125OOTA-7wn5_AidEbJmlKmP926ac3Fmq0Fp42oHTVGvUArSoxupCS_X6IVIUY0lHL1Gr0p5ZYQ2rZCnKATQwiXXK3Qj2uf05iG6PAE-c7nguOMPeTxEZcFBl9wCvg85OhgxpuUi8clurvZl4IfYt7u-ODdEtP8Fr0KMBb_7vCeopuvX35dfGuufl5-vzi_apyQdGla6FTfG9qbFoLuOAMuKRVedgJ6SrSUynRBSdaRIAQA7b2mSrWMeemACH6KPu_n3m-7yffOz0uG0d7nWE94tAmifa7McWOH9GAZM5LR6v948Of0Z-vLYqdYnB9HmH3aFqu0JlQb-V-QCl131ayCYg-6nErJPhyXocTu0rI1LcuFZbamVUvYXVrVd_bvJUfXIZ6qfzjoUByMIcPsYjliTLStYaRieI9t4rD5G7O3UKZnX1bk_R4JkCwMuU65uTaq5a0S_AkNK7KS</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Martinez-Torrecuadrada, J.L. (INGENASA, Madrid.)</creator><creator>Diaz-Laviada, M</creator><creator>Roy, P</creator><creator>Sanchez, C</creator><creator>Vela, C</creator><creator>Sanchez-Vizcaino, J.M</creator><creator>Casal, J.I</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970201</creationdate><title>Serologic markers in early stages of African horse sickness virus infection</title><author>Martinez-Torrecuadrada, J.L. (INGENASA, Madrid.) ; Diaz-Laviada, M ; Roy, P ; Sanchez, C ; Vela, C ; Sanchez-Vizcaino, J.M ; Casal, J.I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c561t-4ab7dd91d94af8b32a36115e6b5ad1086679bf762b0f55aa1de8177422e6ca053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>African Horse Sickness - immunology</topic><topic>African horse sickness virus</topic><topic>African Horse Sickness Virus - immunology</topic><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Antigens, Viral</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Capsid - immunology</topic><topic>Capsid Proteins</topic><topic>Experimental viral diseases and models</topic><topic>INFECCION</topic><topic>INFECTION</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Vaccination</topic><topic>Viral diseases</topic><topic>Viral Proteins - immunology</topic><topic>Viral Vaccines - immunology</topic><topic>VIRUS DE LA PESTE EQUINA AFRICANA</topic><topic>VIRUS PESTE EQUINE AFRICAINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martinez-Torrecuadrada, J.L. (INGENASA, Madrid.)</creatorcontrib><creatorcontrib>Diaz-Laviada, M</creatorcontrib><creatorcontrib>Roy, P</creatorcontrib><creatorcontrib>Sanchez, C</creatorcontrib><creatorcontrib>Vela, C</creatorcontrib><creatorcontrib>Sanchez-Vizcaino, J.M</creatorcontrib><creatorcontrib>Casal, J.I</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martinez-Torrecuadrada, J.L. (INGENASA, Madrid.)</au><au>Diaz-Laviada, M</au><au>Roy, P</au><au>Sanchez, C</au><au>Vela, C</au><au>Sanchez-Vizcaino, J.M</au><au>Casal, J.I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serologic markers in early stages of African horse sickness virus infection</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>35</volume><issue>2</issue><spage>531</spage><epage>535</epage><pages>531-535</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><notes>L73</notes><notes>9743475</notes><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><notes>ObjectType-Article-1</notes><notes>ObjectType-Feature-2</notes><abstract>Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to develop a humoral immune response detectable by immunoblotting. The earliest serological markers corresponded mainly to VP5, VP6, and NS2 and to a lesser extent to VP3, NS1, and NS3. Neutralizing antibodies to VP2 were not detected by immunoblotting, suggesting that they are mostly conformation dependent. VP7-specific antibodies were detected later in infection. These results make NS2 and VP6 the most attractive candidates for the rapid diagnosis of the infection</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>9003637</pmid><doi>10.1128/jcm.35.2.531-535.1997</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0095-1137
ispartof Journal of Clinical Microbiology, 1997-02, Vol.35 (2), p.531-535
issn 0095-1137
1098-660X
language eng
recordid cdi_pubmed_primary_9003637
source Open Access: PubMed Central; American Society for Microbiology Journals
subjects African Horse Sickness - immunology
African horse sickness virus
African Horse Sickness Virus - immunology
Animals
Antibodies, Viral - blood
Antigens, Viral
Biological and medical sciences
Blotting, Western
Capsid - immunology
Capsid Proteins
Experimental viral diseases and models
INFECCION
INFECTION
Infectious diseases
Medical sciences
Vaccination
Viral diseases
Viral Proteins - immunology
Viral Vaccines - immunology
VIRUS DE LA PESTE EQUINA AFRICANA
VIRUS PESTE EQUINE AFRICAINE
title Serologic markers in early stages of African horse sickness virus infection
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T11%3A34%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Serologic%20markers%20in%20early%20stages%20of%20African%20horse%20sickness%20virus%20infection&rft.jtitle=Journal%20of%20Clinical%20Microbiology&rft.au=Martinez-Torrecuadrada,%20J.L.%20(INGENASA,%20Madrid.)&rft.date=1997-02-01&rft.volume=35&rft.issue=2&rft.spage=531&rft.epage=535&rft.pages=531-535&rft.issn=0095-1137&rft.eissn=1098-660X&rft.coden=JCMIDW&rft_id=info:doi/10.1128/jcm.35.2.531-535.1997&rft_dat=%3Cproquest_pubme%3E78801896%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c561t-4ab7dd91d94af8b32a36115e6b5ad1086679bf762b0f55aa1de8177422e6ca053%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=15867982&rft_id=info:pmid/9003637&rfr_iscdi=true