Serologic markers in early stages of African horse sickness virus infection

Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to deve...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 1997-02, Vol.35 (2), p.531-535
Main Authors: Martinez-Torrecuadrada, J.L. (INGENASA, Madrid.), Diaz-Laviada, M, Roy, P, Sanchez, C, Vela, C, Sanchez-Vizcaino, J.M, Casal, J.I
Format: Article
Language:English
Subjects:
African Horse Sickness - immunology
African horse sickness virus
African Horse Sickness Virus - immunology
Animals
Antibodies, Viral - blood
Antigens, Viral
Biological and medical sciences
Blotting, Western
Capsid - immunology
Capsid Proteins
Experimental viral diseases and models
INFECCION
INFECTION
Infectious diseases
Medical sciences
Vaccination
Viral diseases
Viral Proteins - immunology
Viral Vaccines - immunology
VIRUS DE LA PESTE EQUINA AFRICANA
VIRUS PESTE EQUINE AFRICAINE
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Summary:Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to develop a humoral immune response detectable by immunoblotting. The earliest serological markers corresponded mainly to VP5, VP6, and NS2 and to a lesser extent to VP3, NS1, and NS3. Neutralizing antibodies to VP2 were not detected by immunoblotting, suggesting that they are mostly conformation dependent. VP7-specific antibodies were detected later in infection. These results make NS2 and VP6 the most attractive candidates for the rapid diagnosis of the infection
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.35.2.531-535.1997