Identification of an AP‐2 element in the ‐323 to ‐285 region of the TNF‐α gene

We have identified a region of the human TNF‐α promoter between nucleotides ‐323 and ‐285, capable of influencing transcriptional activity. This region encompasses the ‐308 polymorphism and contains a 10 bp sequence homologous to the consensus binding site of activator protein‐2 (AP‐2). Protein comp...

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Bibliographic Details
Published in:Biochemistry and molecular biology international 1996-09, Vol.40 (1), p.43-51
Main Authors: Kroeger, Karen M., Abraham, Lawrence J.
Format: Article
Language:English
Subjects:
DNA-Binding Proteins - chemistry
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation
Humans
Jurkat Cells
Nuclear Proteins - metabolism
Promoter Regions, Genetic
Tetradecanoylphorbol Acetate - pharmacology
Transcription Factor AP-2
Transcription Factors - chemistry
Transfection
Tumor Necrosis Factor-alpha - genetics
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Summary:We have identified a region of the human TNF‐α promoter between nucleotides ‐323 and ‐285, capable of influencing transcriptional activity. This region encompasses the ‐308 polymorphism and contains a 10 bp sequence homologous to the consensus binding site of activator protein‐2 (AP‐2). Protein complexes derived from U937 and Jurkat cells were found to bind to this element. Competitive EMSA using a consensus AP‐2 oligonucleotide indicated that AP‐2 may be involved. Functional assays demonstrate that this region can repress activity of a heterologous promoter in the Jurkat T‐cell line, but act as an inducible enhancer of transcription in U937 cells.
ISSN:1521-6543
1039-9712
1521-6551
DOI:10.1080/15216549600201512