Singlet oxygen (1 delta g) generation from coproporphyrin in Propionibacterium acnes on irradiation

Although singlet oxygen has been postulated to be a highly reactive and toxic intermediate, there has been no evidence of considerable generation of singlet oxygen in vivo level except for special cases. In this work, we firstly measured the near-infrared emission spectra corresponding to the O2(1 d...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-06, Vol.223 (3), p.578
Main Authors: Arakane, K, Ryu, A, Hayashi, C, Masunaga, T, Shinmoto, K, Mashiko, S, Nagano, T, Hirobe, M
Format: Article
Language:English
Subjects:
Coproporphyrins - isolation & purification
Coproporphyrins - radiation effects
Humans
Kinetics
Lasers
Oxygen
Photosensitizing Agents - radiation effects
Propionibacterium acnes - isolation & purification
Propionibacterium acnes - metabolism
Singlet Oxygen
Skin - microbiology
Spectrophotometry, Infrared
Ultraviolet Rays
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Summary:Although singlet oxygen has been postulated to be a highly reactive and toxic intermediate, there has been no evidence of considerable generation of singlet oxygen in vivo level except for special cases. In this work, we firstly measured the near-infrared emission spectra corresponding to the O2(1 delta g) --> O2(3 epsilon g-) transition of singlet oxygen of cutaneous Propionibacterium acnes (P. acnes) porphyrin under laser excitation. A comparison of the singlet oxygen production of coproporphyrin, which is produced predominantly from P. acnes, with that of other photosensitizers revealed coproporphyrin to be a highly efficient singlet oxygen generator under ultraviolet light A irradiation on the skin. These results suggest that singlet oxygen can be generated on the skin surface from P. acnes porphyrin under ultraviolet irradiation and induce serious damage to the skin.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0937