The Saccharomyces cerevisiae SPR1 gene encodes a sporulation-specific exo-1,3-beta-glucanase which contributes to ascospore thermoresistance

A number of genes have been shown to be transcribed specifically during sporulation in Saccharomyces cerevisiae, yet their developmental function is unknown. The SPR1 gene is transcribed during only the late stages of sporulation. We have sequenced the SPR1 gene and found that it has extensive DNA a...

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Bibliographic Details
Published in:Journal of Bacteriology 1993-01, Vol.175 (2), p.386-394
Main Authors: Muthukumar, G. (Scale-Up, Enzon, Inc., Piscataway, NJ), Suhng, S.H, Magee, P.T, Jewell, R.D, Primerano, D.A
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Amino Acid Sequence
Base Sequence
beta-Glucosidase - genetics
beta-Glucosidase - metabolism
Blotting, Southern
CALOR
CHALEUR
DNA, Fungal - genetics
DNA, Fungal - isolation & purification
ESPORAS
GENE
Gene Expression Regulation, Fungal
GENES
Genes, Fungal
GLICOSIDASAS
Glucan 1,3-beta-Glucosidase
GLYCOSIDASE
Hot Temperature
Molecular Sequence Data
NUCLEOTIDE
NUCLEOTIDOS
Open Reading Frames
ORGANE REPRODUCTEUR VEGETAL
ORGANOS REPRODUCTORES VEGETALES
Plasmids
RESISTANCE A LA TEMPERATURE
RESISTENCIA A LA TEMPERATURA
Restriction Mapping
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - physiology
Sequence Homology, Amino Acid
SPORE
Spores, Fungal - physiology
Transcription, Genetic
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Summary:A number of genes have been shown to be transcribed specifically during sporulation in Saccharomyces cerevisiae, yet their developmental function is unknown. The SPR1 gene is transcribed during only the late stages of sporulation. We have sequenced the SPR1 gene and found that it has extensive DNA and protein sequence homology to the S. cerevisiae EXG1 gene which encodes an exo-1,3-beta-glucanase expressed during vegetative growth (C. R. Vasquez de Aldana, J. Correa, P. San Segundo, A. Bueno, A. R. Nebrada, E. Mendez, and F. del Ray, Gene 97:173-182, 1991). We show that spr1 mutant cells do not hydrolyze p-nitrophenyl-beta-D-glucoside or laminarin in a whole-cell assay for exo-1,3-beta-glucanases. In addition to the absence of this enzymatic activity, spr1 mutant spores exhibit reduced thermoresistance relative to isogenic wild-type spores. These observations are consistent with the notion that SPR1 encodes a sporulation-specific exo-1,3-beta-glucanase
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.175.2.386-394.1993