Quantifying platinum binding on protein-functionalized magnetic microparticles using single particle-ICP-TOF-MS

This work describes an analytical procedure, single particle-inductively coupled plasma-time-of-flight-mass spectrometry (SP-ICP-TOF-MS), that was developed to determine the platinum binding efficiency of protein-coated magnetic microparticles. SP-ICP-TOF-MS is advantageous due to its ability to qua...

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Bibliographic Details
Published in:Analytical methods 2024-05, Vol.16 (2), p.3192-321
Main Authors: Bradley, Veronica C, Manard, Benjamin T, Hendriks, Lyndsey, Dunlap, Daniel R, Bible, Amber N, Sedova, Ada, Saint-Vincent, Patricia, Sanders, Brian C, Andrews, Hunter B
Format: Article
Language:English
Subjects:
Azurin
BASIC BIOLOGICAL SCIENCES
Binding
Bulk sampling
Charged particles
Coatings
Differentiation
Digestion
enzyme
Heat treating
ICP-MS
Inductively coupled plasma mass spectrometry
INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Iron
Iron - analysis
Iron - chemistry
Magnetite Nanoparticles - chemistry
Mass spectrometry
Mass Spectrometry - methods
Mass spectroscopy
metal binding
Microparticles
Microspheres
Nuclides
Particle Size
Platinum
Platinum - chemistry
Proteins
Sample preparation
single particle
Streptavidin
Streptavidin - chemistry
time-of-flight
Trace elements
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Summary:This work describes an analytical procedure, single particle-inductively coupled plasma-time-of-flight-mass spectrometry (SP-ICP-TOF-MS), that was developed to determine the platinum binding efficiency of protein-coated magnetic microparticles. SP-ICP-TOF-MS is advantageous due to its ability to quasi-simultaneously detect all nuclides ( 7 Li- 242 Pu), allowing for both platinum and iron (composition of magnetic microparticles) to be measured concurrently. This method subsequently allows for the differentiation between bound and unbound platinum. The 1 μm magnetic microparticles were fully characterized for their iron concentration, particle concentration, and trace element composition by bulk digestion-ICP-MS and SP-ICP-TOF-MS. The results of both approaches agreed with the certificate values. Using the single particle methodology the platinum loading was quantified to be to 0.18 ± 0.02 fg per particle and 0.32 ± 0.02 fg per particle, for the streptavidin-coated and azurin-coated microparticles, respectively. Both streptavidin-coated and the azurin-coated microparticles had a particle-platinum association of >65%. Platinum bound samples were also analyzed via bulk digestion-based ICP-MS. The bulk ICP-MS results overestimated platinum loading due to free platinum in the samples. This highlights the importance of single particle analysis for a closer inspection of platinum binding performance. The SP-ICP-TOF-MS approach offers advantages over typical bulk digestion methods by eliminating laborious sample preparation, enabling differentiation between bound/unbound platinum in a solution, and quantification of platinum on a particle-by-particle basis. The procedure presented here enables quantification of metal content per particle, which could be broadly implemented for other single particle applications. This work describes an analytical procedure, single particle-inductively coupled plasma-time-of-flight-mass spectrometry (SP-ICP-TOF-MS), that was developed to determine the platinum binding efficiency of protein-coated magnetic microparticles.
ISSN:1759-9660
1759-9679
DOI:10.1039/d4ay00268g