Development of a flow-cell bioreactor for immobilized sulfidogenic sludge characterization using electrochemical H 2 S microsensors

The sulfate-reduction process plays a crucial role in the biological valorization of SO gases. However, a complete understanding of the sulfidogenic process in bioreactors is limited by the lack of technologies for characterizing the sulfate-reducing activity of immobilized biomass. In this work, we...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2024-06, Vol.358, p.141959
Main Authors: Castro, Rebeca, Gabriel, Gemma, Gabriel, David, Gamisans, Xavier, Guimerà, Xavier
Format: Article
Language:English
Subjects:
Bacteria - metabolism
Biofilms
Biomass
Bioreactors - microbiology
Electrochemical Techniques - instrumentation
Electrochemical Techniques - methods
Hydrogen Sulfide - analysis
Oxidation-Reduction
Sewage - microbiology
Sulfates - analysis
Sulfates - metabolism
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Summary:The sulfate-reduction process plays a crucial role in the biological valorization of SO gases. However, a complete understanding of the sulfidogenic process in bioreactors is limited by the lack of technologies for characterizing the sulfate-reducing activity of immobilized biomass. In this work, we propose a flow-cell bioreactor (FCB) for characterizing sulfate-reducing biomass using H S microsensors to monitor H S production in real-time within a biofilm. To replace natural immobilization through extracellular polymeric substance production, sulfidogenic sludge was artificially immobilized using polymers. Physical and sulfate-reducing activity studies were performed to select a polymer-biomass matrix that maintained sulfate-reducing activity of biomass while providing strong microbial retention and mechanical strength. Several operational conditions of the sulfidogenic reactor allowed to obtain a H S profiles under different inlet sulfate loads and, additionally, 3D mapping was assessed in order to perform a hydraulic characterization. Besides, the effects of artificial immobilization on biodiversity were investigated through the characterization of microbial communities. This study demonstrated the appropriateness of immobilized-biomass for characterization of sulfidogenic biomass in FCB using H S electrochemical microsensors, and beneficial microbiological communities shifts as well as enrichment of sulfate-reducing bacteria have been confirmed.
ISSN:1879-1298