Deficiency of the BK Ca potassium channel displayed significant implications for the physiology of the human bronchial epithelium

Plasma membrane large-conductance calcium-activated potassium (BK ) channels are important players in various physiological processes, including those mediated by epithelia. Like other cell types, human bronchial epithelial (HBE) cells also express BK in the inner mitochondrial membrane (mitoBK ). T...

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Bibliographic Details
Published in:Mitochondrion 2024-05, Vol.76, p.101880
Main Authors: Maliszewska-Olejniczak, Kamila, Pytlak, Karolina, Dabrowska, Adrianna, Zochowska, Monika, Hoser, Jakub, Lukasiak, Agnieszka, Zajac, Miroslaw, Kulawiak, Bogusz, Bednarczyk, Piotr
Format: Article
Language:English
Subjects:
Bronchi - cytology
Bronchi - metabolism
Cell Line
Cell Membrane - metabolism
CRISPR-Cas Systems
Epithelial Cells - metabolism
Humans
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits - genetics
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits - metabolism
Mitochondria - metabolism
Mitochondrial Membranes - metabolism
Mitochondrial Membranes - physiology
Respiratory Mucosa - cytology
Respiratory Mucosa - metabolism
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Summary:Plasma membrane large-conductance calcium-activated potassium (BK ) channels are important players in various physiological processes, including those mediated by epithelia. Like other cell types, human bronchial epithelial (HBE) cells also express BK in the inner mitochondrial membrane (mitoBK ). The genetic relationships between these mitochondrial and plasma membrane channels and the precise role of mitoBK in epithelium physiology are still unclear. Here, we tested the hypothesis that the mitoBK channel is encoded by the same gene as the plasma membrane BK channel in HBE cells. We also examined the impact of channel loss on the basic function of HBE cells, which is to create a tight barrier. For this purpose, we used CRISPR/Cas9 technology in 16HBE14o- cells to disrupt the KCNMA1 gene, which encodes the α-subunit responsible for forming the pore of the plasma membrane BK channel. Electrophysiological experiments demonstrated that the disruption of the KCNMA1 gene resulted in the loss of BK -type channels in the plasma membrane and mitochondria. We have also shown that HBE ΔαBK cells exhibited a significant decrease in transepithelial electrical resistance which indicates a loss of tightness of the barrier created by these cells. We have also observed a decrease in mitochondrial respiration, which indicates a significant impairment of these organelles. In conclusion, our findings indicate that a single gene encodes both populations of the channel in HBE cells. Furthermore, this channel is critical for maintaining the proper function of epithelial cells as a cellular barrier.
ISSN:1872-8278