Flask and reactor scale production of plant growth regulators by Inonotus hispidus: optimization, immobilization and kinetic parameters

The aims of the presented study are to compare submerged, static, and solid-state fermentation in the production of gibberellic acid (GA 3 ), indole acetic acid (IAA), and abscisic acid (ABA) by Inonotus hispidus, to optimize with a statistical approach, and to determine the kinetic parameters under...

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Published in:Preparative biochemistry & biotechnology 2023-11, Vol.53 (10), p.1210-1223
Main Authors: Doğan, Bilal, Yıldız, Zeki, Aksöz, Nilüfer, Eninanç, Ayşe Betül, Dağ, İlknur, Yıldız, Abdunnasır, Doğan, Hasan Hüseyin, Yamaç, Mustafa
Format: Article
Language:English
Subjects:
Abscisic acid
Acetic acid
Alginates
Alginic acid
Fermentation
Gibberellic acid
Growth hormones
Growth rate
Growth regulators
Immobilization
indole acetic acid
Indoleacetic acid
Inonotus hispidus
Optimization
Parameters
Plant growth
Polyurethane
Polyurethane foam
Reactors
Solid state fermentation
Sustainable agriculture
Synthetic fibers
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Summary:The aims of the presented study are to compare submerged, static, and solid-state fermentation in the production of gibberellic acid (GA 3 ), indole acetic acid (IAA), and abscisic acid (ABA) by Inonotus hispidus, to optimize with a statistical approach, and to determine the kinetic parameters under flask and reactor conditions. The maximum concentrations of GA 3 , (2478.85 ± 68.53 mg/L), ABA, (273.26 ± 6.17 mg/L) and IAA (30.67 ± 0.19 mg/L) were obtained in submerged conditions. After optimization, these values reached 2998.85 ± 28.85, 339.47 ± 5.50, and 34.56 ± 0.25 mg/L, respectively. Immobilization of fungal cells on synthetic fiber, polyurethane foam, and alginate beads resulted in an increase in plant growth regulators (PGR) production by 5.53%− 5.79% under optimized conditions. At the reactor scale, a significant increase was observed for GA 3 concentration, 5441.54 mg/L, which was 2.14 and 1.45 times higher than non-optimized and optimized conditions in the flask scale, respectively. The maximum values for ABA and IAA were 390.39 and 44.79 mg/L, respectively. Although the specific growth rate (µ) decreases relatively from non-optimized flask conditions to optimized reactor conditions, it was observed that the PGR amounts produced per liter medium (r p ) and per gram biomass (Q p ) increased significantly. This is the first report on the synthesis of PGR by Inonotus hispidus which could be crucial for sustainable agriculture.
ISSN:1082-6068
1532-2297
DOI:10.1080/10826068.2023.2185636