1 H, 13 C, 15 N and 31 P chemical shift assignment for stem-loop 4 from the 5'-UTR of SARS-CoV-2

The SARS-CoV-2 virus is the cause of the respiratory disease COVID-19. As of today, therapeutic interventions in severe COVID-19 cases are still not available as no effective therapeutics have been developed so far. Despite the ongoing development of a number of effective vaccines, therapeutics to f...

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Published in:Biomolecular NMR assignments 2021-04
Main Authors: Vögele, Jennifer, Ferner, Jan-Peter, Altincekic, Nadide, Bains, Jasleen Kaur, Ceylan, Betül, Fürtig, Boris, Grün, J Tassilo, Hengesbach, Martin, Hohmann, Katharina F, Hymon, Daniel, Knezic, Bozana, Löhr, Frank, Peter, Stephen A, Pyper, Dennis, Qureshi, Nusrat S, Richter, Christian, Schlundt, Andreas, Schwalbe, Harald, Stirnal, Elke, Sudakov, Alexey, Wacker, Anna, Weigand, Julia E, Wirmer-Bartoschek, Julia, Wöhnert, Jens, Duchardt-Ferner, Elke
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Language:English
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Summary:The SARS-CoV-2 virus is the cause of the respiratory disease COVID-19. As of today, therapeutic interventions in severe COVID-19 cases are still not available as no effective therapeutics have been developed so far. Despite the ongoing development of a number of effective vaccines, therapeutics to fight the disease once it has been contracted will still be required. Promising targets for the development of antiviral agents against SARS-CoV-2 can be found in the viral RNA genome. The 5'- and 3'-genomic ends of the 30 kb SCoV-2 genome are highly conserved among Betacoronaviruses and contain structured RNA elements involved in the translation and replication of the viral genome. The 40 nucleotides (nt) long highly conserved stem-loop 4 (5_SL4) is located within the 5'-untranslated region (5'-UTR) important for viral replication. 5_SL4 features an extended stem structure disrupted by several pyrimidine mismatches and is capped by a pentaloop. Here, we report extensive H, C, N and P resonance assignments of 5_SL4 as the basis for in-depth structural and ligand screening studies by solution NMR spectroscopy.
ISSN:1874-270X