A magnetic surface-enhanced Raman scattering platform for performing successive breast cancer exosome isolation and analysis

The rapid development of exosome research provides new insights into the physiological role of exosomes and their significant correlation with human health. Although the exosomes derived from tumor sources have been proven to be promising biomarkers for cancer detection and disease progression due t...

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Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2021-03, Vol.9 (11), p.279-2716
Main Authors: Li, Guohao, Zhu, Nanhang, Zhou, Juan, Kang, Ke, Zhou, Xiaoxi, Ying, Binwu, Yi, Qiangying, Wu, Yao
Format: Article
Language:English
Subjects:
Biological properties
Biological samples
Biomarkers
Biopsy
Breast cancer
Confidence intervals
Exosomes
Metastases
Pretreatment
Principal components analysis
R&D
Raman spectra
Real time
Research & development
Sensitivity
Tumors
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Summary:The rapid development of exosome research provides new insights into the physiological role of exosomes and their significant correlation with human health. Although the exosomes derived from tumor sources have been proven to be promising biomarkers for cancer detection and disease progression due to their inherited biological contents from the parent cancer cells and unique roles in tumor metastasis and invasion, it is still a challenging task to perform rapid and effective isolation from complex biological samples and conduct high-precision real-time analysis. Herein, we propose a magnetic surface-enhanced Raman scattering (SERS) platform to integrate successive breast cancer exosome isolation and Raman signal enhancement into one system to achieve the goal. In addition, principal component analysis (PCA) was conducted to investigate major patterns of the samples. According to the results, the magnetic SERS platform can be applied to distinguish exosomes derived from MCF-7 and MDA-MB-231 cells with 100% sensitivity and 100% specificity for the 95% confidence interval. More importantly, this platform can fully identify breast cancer patients and healthy people with 91.67% sensitivity and 100% specificity. These studies revealed that our magnetic SERS platform would serve as a great potential system for highly efficient real-time liquid biopsy by using the exosomes as cancerous markers, while exempting from pre-treatment of clinical samples or the extra introduction of elements for SERS signal enhancement. Exosome isolation and SERS are integrated into one system to realize the diagnosis of breast cancer.
ISSN:2050-750X
2050-7518
DOI:10.1039/d0tb02894k