Chronic exposure to ethanol alters the expression of miR-155, miR-122 and miR-217 in alcoholic liver disease in an adult zebrafish model

The aim of this study was to evaluate the hepatic and circulating expression of miR-155, miR-122 and miR-217 in a model of chronic exposure to ethanol in adult zebrafish. Wild-type adult zebrafish were divided into two groups (n = 281): an EG (exposed to 0.5% v/v Ethanol in aquarium water) and a CG...

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Bibliographic Details
Published in:Biomarkers 2021-02, Vol.26 (2), p.146-151
Main Authors: Pasqualotto, Amanda, Ayres, Raquel, Longo, Larisse, Del Duca Lima, Diego, Losch de Oliveira, Diogo, Alvares-da-Silva, Mário Reis, Reverbel da Silveira, Themis, Uribe-Cruz, Carolina
Format: Article
Language:English
Subjects:
Alcoholic liver disease
Animals
Biomarkers - metabolism
Disease Models, Animal
Ethanol
Ethanol - pharmacology
Female
Gene Expression Regulation
Humans
Interleukin-10 - genetics
Interleukin-10 - metabolism
Interleukin-1beta - genetics
Interleukin-1beta - metabolism
Liver - drug effects
Liver - metabolism
Liver Diseases, Alcoholic - genetics
Liver Diseases, Alcoholic - metabolism
Liver Diseases, Alcoholic - pathology
Male
MicroRNAs - genetics
MicroRNAs - metabolism
miR-122
miR-155
miR-217
Triglycerides - metabolism
Tumor Necrosis Factor-alpha - genetics
Tumor Necrosis Factor-alpha - metabolism
Zebrafish
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Summary:The aim of this study was to evaluate the hepatic and circulating expression of miR-155, miR-122 and miR-217 in a model of chronic exposure to ethanol in adult zebrafish. Wild-type adult zebrafish were divided into two groups (n = 281): an EG (exposed to 0.5% v/v Ethanol in aquarium water) and a CG (without ethanol). After 28 days the animals were euthanized, followed by histopathological analysis, quantification of lipids, triglycerides and inflammatory cytokines in liver tissue. miR-155, miR-122 and miR-217 gene expression was quantified in liver tissue and serum. We observed hepatic lesions and increased accumulation of hepatic lipids in the EG. The expression of il-1β was higher in the EG, but there were no differences in il-10 and tnf-α between groups. In the liver, expression of miR-122 and miR-155 was higher in the EG. The circulating expression of miR-155 and miR-217 was significantly higher in the EG. Chronic exposure to ethanol in zebrafish leads to altered hepatic and circulating expression of miR-155, miR-122 and miR-217. This confirms its potential as a biomarker and therapeutic target.
ISSN:1354-750X
1366-5804
DOI:10.1080/1354750X.2021.1874051