Key mutations in the C-terminus of the HBV surface glycoprotein correlate with lower HBsAg levels in vivo, hinder HBsAg secretion in vitro and reduce HBsAg structural stability in the setting of HBeAg-negative chronic HBV genotype-D infection

Increasing evidences suggest that HBsAg-production varies across HBV-genotypes. HBsAg C-terminus plays a crucial role for HBsAg-secretion. Here, we evaluate HBsAg-levels in different HBV-genotypes in HBeAg-negative chronic infection, the correlation of specific mutations in HBsAg C-terminus with HBs...

Full description

Saved in:
Bibliographic Details
Published in:Emerging microbes & infections 2020-01, Vol.9 (1), p.928-939
Main Authors: Salpini, Romina, Battisti, Arianna, Piermatteo, Lorenzo, Carioti, Luca, Anastasiou, Olympia E., Gill, Upkar S., Di Carlo, Domenico, Colagrossi, Luna, Duca, Leonardo, Bertoli, Ada, La Rosa, Katia Yu, Fabeni, Lavinia, Iuvara, Alessandra, Malagnino, Vincenzo, Cerva, Carlotta, Lichtner, Miriam, Mastroianni, Claudio M., De Sanctis, Giuseppe Maria, Paoloni, Maurizio, Marignani, Massimo, Pasquazzi, Caterina, Iapadre, Nerio, Parruti, Giustino, Vecchiet, Jacopo, Sarmati, Loredana, Andreoni, Massimo, Angelico, Mario, Grelli, Sandro, T. Kennedy, Patrick, Verheyen, Jens, Aquaro, Stefano, Ceccherini-Silberstein, Francesca, Perno, Carlo Federico, Svicher, Valentina
Format: Article
Language:English
Subjects:
Adult
Female
Genotype
Genotype & phenotype
HBeAg-negative infection
HBsAg C-terminus
HBsAg levels
HBsAg mutations
HBV genotypes
Hepatitis B Surface Antigens - analysis
Hepatitis B Surface Antigens - genetics
Hepatitis B virus - genetics
Hepatitis B virus - pathogenicity
Hepatitis B, Chronic - diagnosis
Humans
Infections
Male
Middle Aged
Mutation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Increasing evidences suggest that HBsAg-production varies across HBV-genotypes. HBsAg C-terminus plays a crucial role for HBsAg-secretion. Here, we evaluate HBsAg-levels in different HBV-genotypes in HBeAg-negative chronic infection, the correlation of specific mutations in HBsAg C-terminus with HBsAg-levels in-vivo, their impact on HBsAg-secretion in-vitro and on structural stability in-silico. HBsAg-levels were investigated in 323 drug-naïve HBeAg-negative patients chronically infected with HBV genotype-D(N = 228), -A(N = 65) and -E(N = 30). Genotype-D was characterized by HBsAg-levels lower than genotype-A and -E (3.3[2.7-3.8]IU/ml; 3.8[3.5-4.2]IU/ml and 3.9[3.7-4.2]IU/ml, P 
ISSN:2222-1751
2222-1751
DOI:10.1080/22221751.2020.1757998