Genomic Organization of the Human T-Cell Antigen-Receptor α /δ Locus

Two clusters of overlapping cosmid clones comprising about 100 kilobases (kb) at the human T-cell antigen-receptor α /δ locus were isolated from a genomic library. The structure of the germ-line Vδ1 variable gene segment was determined. Vδ1 is located 8.5 kb downstream of the Vα13.1 gene segment, an...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-11, Vol.85 (21), p.8166-8170
Main Authors: Satyanarayana, Karuturi, Hata, Shingo, Devlin, Peter, Roncarolo, Maria Grazia, De Vries, Jan E., Spits, Hergen, Strominger, Jack L., Krangel, Michael S.
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Cell lines
Chromosome Inversion
Chromosomes
Complementary DNA
Cosmids
DNA
DNA probes
Electrophoresis
Fundamental and applied biological sciences. Psychology
Genes
Genes. Genome
Genetic Linkage
Genetic loci
Genomics
Humans
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleotide Mapping
Receptors, Antigen, T-Cell - genetics
T cell antigen receptors
Transcription, Genetic
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Summary:Two clusters of overlapping cosmid clones comprising about 100 kilobases (kb) at the human T-cell antigen-receptor α /δ locus were isolated from a genomic library. The structure of the germ-line Vδ1 variable gene segment was determined. Vδ1 is located 8.5 kb downstream of the Vα13.1 gene segment, and both V segments are arranged in the same transcriptional orientation. The Vα17.1 segment is located between Vδ1 and the Dδ, Jδ, Cδ region (containing the diversity, joining, and constant gene segments). Thus, Vδ and Vα segments are interspersed along the chromosome. The germ-line organization of the Dδ2, Jδ1, and Jδ2 segments was determined. Linkage of Cδ to the Jα region was established by identification of Jα segments within 20 kb downstream of Cδ. The organization of the locus was also analyzed by field-inversion gel electrophoresis. The unrearranged Vδ1 and Dδ, Jδ, Cδ regions are quite distant from each other, apparently separated by a minimum of 175-180 kb.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.21.8166