Evaluation of biological activities and in vivo amelioration of CCl 4 induced toxicity in lung and kidney with Abutilon pannosum (G.Forst.) Schltdl. in rat

Abutilon pannosum is used in Pakistan for bladder inflammation, diuretic, lung disorders, diabetes and in lowering pyrexia. Amount of total phenolic content, total flavonoid content and HPLC analysis of APM for the presence of polyphenolics were carried out. Antioxidant activity was determined by us...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2020-03, Vol.249, p.112395
Main Authors: Khalil, Iza, Ghani, Marvi, Khan, Muhammad Rashid, Akbar, Fakhrah
Format: Article
Language:English
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Summary:Abutilon pannosum is used in Pakistan for bladder inflammation, diuretic, lung disorders, diabetes and in lowering pyrexia. Amount of total phenolic content, total flavonoid content and HPLC analysis of APM for the presence of polyphenolics were carried out. Antioxidant activity was determined by using different in vitro antioxidant assays. Amelioration effects of APM (200 mg/kg body weight and 400 mg/kg body weight) against CCl induced kidney and lung toxicity in rat was assessed by determining level of antioxidant enzymes, lipid peroxidation products, cytokines (TNF-α, IL-1β and IL-2), comet assay and histological analysis. HPLC-DAD studies of APM indicated the presence of rutin (0.635 ± 0.011 μg/mg dry extract), gallic acid (1.07 ± 0.043 μg/mg dry extract) and catechin (0.246 ± 0.08 μg/mg dry extract) and considerable quantity of total phenolic (55.485 ± 0.85 mg GAE/g dry extract) and total flavonoid content (19.90 ± 0.58 mg Rutin/g dry extract). During in vitro antioxidant assays APM showed significant (p 
ISSN:1872-7573