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The Role of Iron in the P-Acquisition Mechanisms of the Unicellular N 2 -Fixing Cyanobacteria Halothece sp., Found in Association With the Mediterranean Seagrass Posidonia oceanica
, an endemic seagrass of the Mediterranean Sea harbors a high diversity of N -fixing prokaryotes. One of these is sp., a unicellular N -fixing cyanobacteria detected through analysis from the epiphytes of . . The most related strain in culture is sp. PCC 7418 and this was used as the test organism i...
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| Published in: | Frontiers in microbiology 2019-08, Vol.10, p.1903 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Online Access: | Get full text |
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| Summary: | , an endemic seagrass of the Mediterranean Sea harbors a high diversity of N
-fixing prokaryotes. One of these is
sp., a unicellular N
-fixing cyanobacteria detected through
analysis from the epiphytes of
.
. The most related strain in culture is
sp. PCC 7418 and this was used as the test organism in this study. In the Mediterranean Sea, phosphorus (P) and iron (Fe) can be the major limiting nutrients for N
fixation. However, information about the mechanisms of P-acquisition and the role of metals (i.e., Fe) in these processes for N
-fixing bacteria is scarce. From our genomic analyses of the test organism and other phylogenetically related N
-fixing strains,
sp. PCC 7418 is one of the strains with the greatest number of gene copies (eight copies) of alkaline phosphatases (APases). Our structural analysis of PhoD (alkaline phosphatase type D) and PhoU (phosphate acquisition regulator) of
sp. PCC 7418 showed the connection among metals (Ca
and Fe
), and the P-acquisition mechanisms. Here, we measured the rates of alkaline phosphatase activity (APA) through MUF-P hydrolysis under different combinations of concentrations of inorganic P (
) and Fe in experiments under N
-fixing (low
availability) and non-N
fixing (high
availability) conditions. Our results showed that APA rates were enhanced by the increase in Fe availability under low levels of
, especially under N
-fixing conditions. Moreover, the increased
-uptake was reflected in the increased of the P-cellular content of the cells under N
fixation conditions. We also found a positive significant relationship between cellular P and cellular Fe content of the cells (
= 0.71,
< 0.05). Our results also indicated that Fe-uptake in
sp. PCC 7418 was P and Fe-dependent. This study gives first insights of P-acquisition mechanisms in the N
-fixing cyanobacteria (
sp.) found in
.
and highlights the role of Fe in these processes. |
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| ISSN: | 1664-302X 1664-302X |
| DOI: | 10.3389/fmicb.2019.01903 |