Comment on "Quality of extracellular vesicle images by transmission electron microscopy is operator and protocol dependent"

[...]current efforts towards the development of optimized preparation protocols for EV imaging by negative staining EM, as presented in Rikkert and colleagues' article, are highly valuable. [...]in the context of this article, a major advantage of cryo-EM over negative staining deserves to be e...

Full description

Saved in:
Bibliographic Details
Published in:Journal of extracellular vesicles 2019-12, Vol.8 (1), p.1648996-n/a
Main Author: Brisson, Alain R.
Format: Article
Language:English
Subjects:
Carbon
Chemical Sciences
Extracellular vesicles
Letter to the Editor
Material chemistry
Morphology
Protocol
Stains & staining
Transmission electron microscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[...]current efforts towards the development of optimized preparation protocols for EV imaging by negative staining EM, as presented in Rikkert and colleagues' article, are highly valuable. [...]in the context of this article, a major advantage of cryo-EM over negative staining deserves to be emphasized. The absence of adsorption issue correlates with the highly homogeneous distribution of objects, which characterizes cryo-EM grids [9]. [...]in the case of cryo-EM, it is forseeable that imaging EV samples at predefined positions, say, in a fully automated manner or with minimal intervention from the operator, will become a routine approach.
ISSN:2001-3078
2001-3078
DOI:10.1080/20013078.2019.1648996