SOX13 dependent PAX8 expression promotes the proliferation of gastric carcinoma cells

PAX8 is identified as a regulator in the pathogenesis of human tumours and an indicator of the prognosis for patients. However, the role of PAX8 on proliferation in gastric cancer have not been studied. This study was aimed to explore the expression pattern of PAX8 in gastric cancer, and investigate...

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Published in:Artificial cells, nanomedicine, and biotechnology nanomedicine, and biotechnology, 2019-12, Vol.47 (1), p.3180-3187
Main Authors: Bie, Liang-Yu, Li, Dan, Wei, Yan, Li, Ning, Chen, Xiao-Bing, Luo, Su-Xia
Format: Article
Language:English
Subjects:
Apoptosis
Aurora B protein
Autoantigens - metabolism
Cancer
Cell cycle
Cell Line, Tumor
Cell Proliferation
Cyclin B1
expression
Flow cytometry
G1 phase
Gastric cancer
gastric carcinoma
Gene expression
Gene Expression Regulation, Neoplastic
Humans
Pathogenesis
PAX8
Pax8 protein
PAX8 Transcription Factor - genetics
Prognosis
proliferation
SOX13
SOXD Transcription Factors - metabolism
Stomach Neoplasms - diagnosis
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Survival Analysis
Tumors
Up-Regulation
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Summary:PAX8 is identified as a regulator in the pathogenesis of human tumours and an indicator of the prognosis for patients. However, the role of PAX8 on proliferation in gastric cancer have not been studied. This study was aimed to explore the expression pattern of PAX8 in gastric cancer, and investigate the effect of PAX8 on the proliferation of gastric cancer cells. PAX8 and SOX13 were identified to be synchronously up-regulated in primary gastric cancer in human gastric cancer tissues and the gastric cancer datasets of TCGA, and gastric cancer patients of combined high PAX8 and SOX13 expression showed poor prognosis. Furthermore, SOX13 can mediate PAX8 and its targeted genes, Aurora B and Cyclin B1, expression in AGS and MGC803 cell lines. Flow cytometry and EdU incorporation assays showed that silencing PAX8 can block the cell cycle of gastric cancer cell in G1 phase and SOX13 expression can rescue the arrested proliferative process induced by PAX8 silenced in CCK8 and colony formation assays. Thus, combined SOX13 and PAX8 expression regulate the proliferation of gastric cancer cells, and both SOX13 and PAX8 play an oncogene function in gastric cancer.
ISSN:2169-1401
2169-141X
DOI:10.1080/21691401.2019.1646751