A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase

We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous...

Full description

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2019-07, Vol.55 (53), p.7611-7614
Main Authors: Zhang, Yu-Peng, Cui, Yun-Xi, Li, Xiao-Yu, Du, Yi-Chen, Tang, An-Na, Kong, De-Ming
Format: Article
Language:English
Subjects:
Amplification
Biosensing Techniques
Biosensors
Design modifications
HeLa Cells
Humans
Kinases
Nucleic Acid Amplification Techniques
Polynucleotide 5'-Hydroxyl-Kinase - analysis
Polynucleotide 5'-Hydroxyl-Kinase - metabolism
Signal to noise ratio
Spectrometry, Fluorescence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous PNK activity at the single-cell level. We reported a modified exponential amplification reaction strategy and applied it to design an ultrasensitive biosensor for the detection of endogenous polynucleotide kinase activity at single-cell level.
ISSN:1359-7345
1364-548X
DOI:10.1039/c9cc03568k