Number and brightness analysis to study spatio-temporal distribution of the angiotensin II AT 1 and the endothelin-1 ET A receptors: Influence of ligand binding

The angiotensin II AT and the endothelin 1 ET receptors play a crucial role in the pathogenesis of cardiovascular diseases like hypertension, heart failure, stroke, pulmonary hypertension, and cardiac hypertrophy. Both receptors are members of the rhodopsion-like superfamily of G protein-coupled rec...

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Published in:Biochimica et biophysica acta. General subjects 2019-05, Vol.1863 (5), p.917
Main Authors: Planes, Nadir, Digman, Michelle A, Vanderheyden, Patrick P M L, Gratton, Enrico, Caballero-George, Catherina
Format: Article
Language:English
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Summary:The angiotensin II AT and the endothelin 1 ET receptors play a crucial role in the pathogenesis of cardiovascular diseases like hypertension, heart failure, stroke, pulmonary hypertension, and cardiac hypertrophy. Both receptors are members of the rhodopsion-like superfamily of G protein-coupled receptors which can exist as monomers, dimers, and higher order aggregates. Recently, oligomerization of these two receptors have been described by several biophysical methods based mainly on luminescence and fluorescence energy transfer. Since this oligomerization can occur either spontaneously or it can be induced by ligand-binding, the aim of this work was to address whether the oligomerization of these receptors occurs upon ligand-binding. For this purpose the Number and Brightness analysis, a method that allows the identification, localization, and quantification of protein aggregates in the plasma membrane of a single cell, was used. An advantage of this method is that it is not limited to certain dyes specially required for Fluorescence Resonance Energy Transfer measurements. Our results showed that stably transfected angiotensin II AT receptors and transiently transfected endothelin 1 ET receptors, were found as monomeric, dimeric, and tetrameric receptor aggregates. Interestingly, the binding of antihypertensive agents like losartan and BQ123, earlier suggested to be inverse agonists, significantly increased the proportion of monomers and reduced the occurrence of dimers on the cell membrane; while the kown endothelin 1 ET antagonist sitaxentan did not influence the aggregation state of these receptors.
ISSN:1872-8006
DOI:10.1016/j.bbagen.2019.03.004