Influence of Age on Induction of Mammary Tumors by Diethylstilbestrol in C3H/HeN Mice With Low Murine Mammary Tumor Virus Titer

C3H/HeN female mice with low murine mammary tumor virus titer (MTV−) were fed diets containing a targeted concentration of 640 ppb diethylstilbestrol [(DES) CAS: 56-53-1; 4,4′-(1,2-diethyl-1,2-ethenediyl)bis-phenol]. Mice were started on DES at 3, 5, 7, or 9 weeks of age. Some continued on the diet...

Full description

Saved in:
Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1986-10, Vol.77 (4), p.891-898
Main Authors: Greenman, David L., Highman, Benjamin, Chen, James J., Schieferstein, George J., Norvell, Michael J.
Format: Article
Language:English
Subjects:
Adenocarcinoma - chemically induced
Adenocarcinoma - microbiology
Adenocarcinoma - pathology
Aging
Animal tumors. Experimental tumors
Animals
Biological and medical sciences
Corpus Luteum - pathology
Diethylstilbestrol
Experimental genital and mammary tumors
Female
Granulosa Cell Tumor - chemically induced
Mammary Neoplasms, Experimental - chemically induced
Mammary Neoplasms, Experimental - microbiology
Mammary Neoplasms, Experimental - pathology
Mammary Tumor Virus, Mouse - isolation & purification
Medical sciences
Mice
Mice, Inbred C3H
Ovarian Neoplasms - chemically induced
Pituitary Gland - pathology
Pituitary Neoplasms - chemically induced
Thecoma - chemically induced
Tumors
Uterine Neoplasms - chemically induced
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:C3H/HeN female mice with low murine mammary tumor virus titer (MTV−) were fed diets containing a targeted concentration of 640 ppb diethylstilbestrol [(DES) CAS: 56-53-1; 4,4′-(1,2-diethyl-1,2-ethenediyl)bis-phenol]. Mice were started on DES at 3, 5, 7, or 9 weeks of age. Some continued on the diet throughout the rest of their life-spans, whereas others were killed as soon as they had been fed DES for 2, 4, 6, 8, 10, or 12 weeks. Controls were also examined throughout the study. Among mice killed early, the only observation significantly influenced by age at the start of DES treatment was the presence or absence of corpora lutea (CL). DES did not prevent CL from appearing in mice started on DES at 7 or 9 weeks of age, but it did prevent their appearance in about 25% of the mice started at 5 weeks and in up to 75% of the mice started at 3 weeks of age. In the life-span-exposure groups, CL either disappeared or were never formed in 88% or more of the mice, regardless of age at the start of treatment. Neoplastic or presumptive preneoplastic lesions apparently influenced by DES in the life-span-treatment groups included ovarian tubular adenomas; granulosa cell tumors and luteomas; pituitary cystoid degeneration, hyperplasia, and adenomas; uterine adenocarcinomas and cervical adenosis; mesotheliomas; and mammary hyperplastic alveolar nodules (HANs) and adenocarcinomas. Luteoma and granulosa cell tumor incidences were reduced by DES, regardless of age at the start of treatment. Influence of age at the start of treatment was minimal or not apparent for mesotheliomas, uterine adenocarcinomas, or pituitary adenomas; however, pituitary cystoid degeneration and hyperplasia and cervical adenosis occurred in higher frequency and/or with shorter duration of DES exposure the earlier that treatment was started. A delay in the start of DES treatment was associated with a remarkable delay in HAN and mammary adenocarcinoma development. This was especially apparent in young mice (3–7 wk old) in which a 2-week delay in treatment resulted in a 20-week delay in HAN or tumor onset. Age at the start of treatment was a major factor in susceptibility of C3H/HeN-MTV− female mice to DES-induced mammary tumori-genesis.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/77.4.891