Gene expression of an arabinogalactan lysine-rich protein Ca AGP18 during vegetative and reproductive development of bell pepper ( Capsicum annuum L.)

Lysine-rich (Lys-rich) proteins encoded by , , and genes are cell wall-associated glycopeptides related to sexual reproduction in flowering plants. This subclass belongs to classical arabinogalactan proteins (AGPs) widely studied in model plants like . In this study, we identified the cDNA from bell...

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Published in:3 Biotech 2018-01, Vol.8 (1), p.5
Main Authors: Verdugo-Perales, Mercedes, Velez-de la Rocha, Rosabel, León-Félix, Josefina, Osuna-Enciso, Tomas, Heredia, José B, Osuna-Castro, Juan A, Islas-Osuna, Maria A, Sañudo-Barajas, J Adriana
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Language:English
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Summary:Lysine-rich (Lys-rich) proteins encoded by , , and genes are cell wall-associated glycopeptides related to sexual reproduction in flowering plants. This subclass belongs to classical arabinogalactan proteins (AGPs) widely studied in model plants like . In this study, we identified the cDNA from bell pepper ( L.), as well as its expression pattern during vegetative and reproductive development. The deduced amino acid sequence revealed a Lys-rich AGP18 protein of 238 amino acids residues in length with an estimated molecular mass of 22.85 kDa and an isoelectric point of 9.7. The protein is predicted as canonical AGP due to the presence of a small Lys-rich region and a C-terminal sequence essential for posttranslational modification with a glycosylphosphatidylinositol (GPI). Phylogenetic analysis showed that is clustered together with , , and from species. expression through plant phenological stages had the highest transcription level in leaves at the seedling stage, whereas in reproductive organs there was a significant up-regulation in pistils during anthesis, also in petals 2 days post-anthesis (DPA), and in fruit at the expansion stage. Our results open future research for possible roles of AGP18 in cell expansion as a wall-associated plasticizer and reproductive processes like pistil interactions and petal cell death.
ISSN:2190-572X