A new instrument of VUV laser desorption/ionization mass spectrometry imaging with micrometer spatial resolution and low level of molecular fragmentation

Mass spectrometry imaging (MSI) has important applications in material research, biology, and medicine. The MSI method based on UV laser desorption/ionization (UVLDI) can obtain images of intact samples, but has a high level of molecular fragmentation. In this work, we report a new MSI instrument th...

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Bibliographic Details
Published in:Review of scientific instruments 2017-11, Vol.88 (11), p.114102-114102
Main Authors: Wang, Jia, Liu, Feng, Mo, Yuxiang, Wang, Zhaoying, Zhang, Sichun, Zhang, Xinrong
Format: Article
Language:English
Subjects:
Ablation
Brain
Collimation
Desorption
Fibrinogen
Four-wave mixing
Fragmentation
Ionization
Ions
Laser beam heating
Lasers
Low level
Mass spectra
Mass spectrometry
Scientific apparatus & instruments
Scientific imaging
Spatial resolution
Spectroscopy
Ultraviolet lasers
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Summary:Mass spectrometry imaging (MSI) has important applications in material research, biology, and medicine. The MSI method based on UV laser desorption/ionization (UVLDI) can obtain images of intact samples, but has a high level of molecular fragmentation. In this work, we report a new MSI instrument that uses a VUV laser (125.3 nm) as a desorption/ionization source to exploit its advantages of high single photon energy and small focus size. The new instrument was tested by the mass spectra of Nile red and FGB (Fibrinogen beta chain) samples and mass spectrometric images of a fly brain section. For the tested samples, the VUVDI method offers lower levels of molecular fragmentations and higher sensitivities than those of the UVLDI method and second ion mass spectrometry imaging method using a Bi 3 + beam. The ablation crater produced by the focused VUV laser on a quartz plate has an area of 10 μm2. The VUV laser is prepared based on the four-wave mixing method using three collimated laser beams and a heated Hg cell.
ISSN:0034-6748
1089-7623
DOI:10.1063/1.4994173