Sequencing of bla IMP -Carrying IncN2 Plasmids, and Comparative Genomics of IncN2 Plasmids Harboring Class 1 Integrons

This work presents the complete nucleotide sequences of p0801-IMP from , p7121-IMP from , and p17285-IMP from , which are recovered from three different cases of nosocomial infection. These three plasmids represent the first fully sequenced -carrying IncN2 plasmids. Further comparative genomics anal...

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Bibliographic Details
Published in:Frontiers in cellular and infection microbiology 2017, Vol.7, p.102
Main Authors: Jiang, Xiaoyuan, Yin, Zhe, Yin, Xiuyun, Fang, Haihong, Sun, Qiang, Tong, Yigang, Xu, Yuanhong, Zhang, Defu, Feng, Jiao, Chen, Weijun, Song, Yajun, Wang, Jinglin, Chen, Shuiping, Zhou, Dongsheng
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
beta-Lactamases - genetics
beta-Lactamases - metabolism
Gene Order
Genome
Genomics - methods
Integrons - genetics
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - genetics
Microbial Sensitivity Tests
Plasmids - genetics
Sequence Analysis, DNA
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Summary:This work presents the complete nucleotide sequences of p0801-IMP from , p7121-IMP from , and p17285-IMP from , which are recovered from three different cases of nosocomial infection. These three plasmids represent the first fully sequenced -carrying IncN2 plasmids. Further comparative genomics analysis of all the five integron-carrying IncN2 plasmids p0801-IMP, p7121-IMP, p17285-IMP, pJIE137, and p34983-59.134kb indicates that they possess conserved IncN2 backbones with limited genetic variations with respect to gene content and organization. Four class 1 integrons ( -carrying In1223 in p0801-IMP/p7121-IMP, -carrying In655 in p17285-IMP, In27 in pJIE137, and In1130 in p34983-59.134kb), two insertion sequence-based transposition units (IS - in p17285-IMP, and IS - -Δ - in pJIE137), and a novel Tn -related transposon Tn carrying In1130 in p34983-59.134kb are indentified in the plasmid accessory regions. In1223 and In655 represent ancestral Tn -associated integrons, while In27 and In1130 belong to complex class 1 integrons. The relatively small IncN2 backbones are able to integrate different mobile elements which carry various resistance markers, promoting the accumulation and spread of antimicrobial resistance genes among enterobacterial species.
ISSN:2235-2988