In vitro DNA damage by Casiopeina II-gly in human blood cells

A variety of metal ions have biological functions, and many researchers have not actively investigated copper compounds, based on the assumption that endogenous metals might be less toxic. In the present study, we used a dual fluorochrome method and a single cell gel electrophoresis (SCGE) assay at...

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Published in:Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 2017-04, Vol.40 (2), p.164-170
Main Authors: Rodríguez-Mercado, Juan José, Florín-Ramírez, Diana, Álvarez-Barrera, Lucila, Altamirano-Lozano, Mario Agustín
Format: Article
Language:English
Subjects:
Casiopeina II-gly
Cell Survival - drug effects
Comet Assay
Copper-based antineoplastic drug
cytotoxicity
DNA damage
Dose-Response Relationship, Drug
Female
genotoxicity
Humans
Leukocytes - drug effects
Leukocytes - pathology
Male
Middle Aged
Mutagens - toxicity
Organometallic Compounds - toxicity
Risk Assessment
SCGE in human leucocytes
Time Factors
Young Adult
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Summary:A variety of metal ions have biological functions, and many researchers have not actively investigated copper compounds, based on the assumption that endogenous metals might be less toxic. In the present study, we used a dual fluorochrome method and a single cell gel electrophoresis (SCGE) assay at pH > 13 to evaluate the cell viability and DNA damage induced by a copper-based antineoplastic drug, Casiopeina II-gly®, at concentrations of 1.04, 2.08, 4.17, 8.35 or 16 μg/mL in human peripheral-blood leukocytes in vitro. We observed that Casiopeina II-gly® reduced cell viability at high concentrations (8.35 and 16 μg/mL) and induced DNA damage characterized by single-strand breaks and alkali labile sites at several concentrations from 2.08 to 16 μg/mL. This chemical clearly affected DNA migration in a concentration- and time-dependent manner and induced genotoxic effects in few minutes (>20 min), at which point the genotoxicity was followed by cytotoxicity.
ISSN:0148-0545
1525-6014
DOI:10.1080/01480545.2016.1190738