In vitro and in vivo antimicrobial efficacy of natural plant-derived compounds against Vibrio cholerae of O1 El Tor Inaba serotype

In this study, we investigated antibacterial activities of 20 plant-derived natural compounds against Gram-negative enteric pathogens. We found that both flavonoids and non-flavonoids, including honokiol and magnolol, possess specific antibacterial activities against V. cholerae, but not against oth...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2015-03, Vol.79 (3), p.475-483
Main Authors: Kim, Hyung-Ip, Kim, Ji-Ae, Choi, Eun-Jin, Harris, Jason B., Jeong, Seong-Yeop, Son, Seok-Jun, Kim, Younghoon, Shin, Ok Sarah
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
Anti-Inflammatory Agents - pharmacology
Anti-Inflammatory Agents - therapeutic use
antibacterial effect
Biofilms - drug effects
Biological Products - pharmacology
Biological Products - therapeutic use
C. elegans
Caenorhabditis elegans
Cholera - drug therapy
Food
honokiol
Lipopolysaccharides - adverse effects
Macrophages - drug effects
magnolol
Mice
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
RAW 264.7 Cells
V. cholerae
Vibrio cholerae - drug effects
Vibrio cholerae - physiology
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Summary:In this study, we investigated antibacterial activities of 20 plant-derived natural compounds against Gram-negative enteric pathogens. We found that both flavonoids and non-flavonoids, including honokiol and magnolol, possess specific antibacterial activities against V. cholerae, but not against other species of Gram-negative bacterium which we tested. Using various antibacterial assays, we determined that there was a dose-dependent bactericidal and biofilm inhibitory activity of honokiol and magnolol against Vibrio cholerae. In addition to antibacterial activities, these molecules also induced an attenuating effect on reactive oxygen species (ROS) production and pro-inflammatory responses generated by macrophages in response to lipopolysaccharides (LPS). Additionally, Caenorhabditis elegans lethality assay revealed that honokiol and magnolol have an ability to extend a lifespan of V. cholerae-infected worms, contributing to prolonged survival of worms after lethal infection. Altogether, our data show for the first time that honokiol and magnolol may be considered as attractive protective or preventive food adjuncts for cholera. Effect of honokiol and magnolol on ROS production in V. cholerae LPS-stimulated mouse macrophages (in vitro) and on the survivals of C. elegans host (in vivo)
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2014.991685