A comparative study on collagen type I and hyaluronic acid dependent cell behavior for osteochondral tissue bioprinting

Bioprinting is a promising technique for engineering composite tissues, such as osteochondral tissues. In this study, as a first step toward bioprinting-based osteochondral tissue regeneration, we systematically examined the behavior of chondrocytes and osteoblasts to hyaluronic acid (HA) and type I...

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Published in:Biofabrication 2014-09, Vol.6 (3), p.035004-11
Main Authors: Park, Ju Young, Choi, Jong-Cheol, Shim, Jin-Hyung, Lee, Jung-Seob, Park, Hyoungjun, Kim, Sung Won, Doh, Junsang, Cho, Dong-Woo
Format: Article
Language:English
Subjects:
Biocompatibility
Biocompatible Materials - chemistry
Biomedical materials
bioprinting
Bioprinting - instrumentation
Bioprinting - methods
Cell Differentiation
cell migration
Chondrocytes - cytology
Collagen Type I - chemistry
Collagens
Electrochemical machining
extracellular matrix
Humans
Hyaluronic Acid - chemistry
Hydrogels
Hydrogels - chemistry
Hydroxyapatite
Osteoblasts
Osteoblasts - cytology
osteochondral tissue
Three dimensional
tissue engineering
Tissue Engineering - instrumentation
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:Bioprinting is a promising technique for engineering composite tissues, such as osteochondral tissues. In this study, as a first step toward bioprinting-based osteochondral tissue regeneration, we systematically examined the behavior of chondrocytes and osteoblasts to hyaluronic acid (HA) and type I collagen (Col-1) hydrogels. First, we demonstrated that cells on hydrogels that were comprised of major native tissue extracellular matrix (ECM) components (i.e. chondrocytes on HA hydrogels and osteoblasts on Col-1 hydrogels) exhibited better proliferation and cell function than cells on non-native ECM hydrogels (i.e., chondrocytes on Col-1 hydrogels and osteoblasts on HA hydrogels). In addition, cells located near their native ECM hydrogels migrated towards them. Finally, we bioprinted three-dimensional (3D) osteochondral tissue-mimetic structures composed of two compartments, osteoblast-encapsulated Col-1 hydrogels and chondrocyte-encapsulated HA hydrogels, and found viability and functions of each cell type were well maintained within the 3D structures up to 14 days in vitro. These results suggest that with proper choice of hydrogel materials, bioprinting-based approaches can be successfully applied for osteochondral tissue regeneration.
ISSN:1758-5082
1758-5090
DOI:10.1088/1758-5082/6/3/035004