Non-transferrin-bound iron in plasma or serum from patients with idiopathic hemochromatosis. Characterization by high performance liquid chromatography and nuclear magnetic resonance spectroscopy

The nature of non-transferrin-bound iron in the plasma or serum of iron-overloaded hemochromatosis patients was studied by high performance liquid chromatography (HPLC) and high resolution nuclear magnetic resonance (NMR). 500-MHz proton Hahn spin-echo NMR spectra of plasma or serum, combined with t...

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Bibliographic Details
Published in:The Journal of biological chemistry 1989-03, Vol.264 (8), p.4417
Main Authors: Grootveld, M, Bell, J D, Halliwell, B, Aruoma, O I, Bomford, A, Sadler, P J
Format: Article
Language:English
Subjects:
Acetates - blood
Bleomycin
Chlorides
Chromatography, High Pressure Liquid
Citrates - blood
Citric Acid
Deferoxamine
Ferric Compounds
Hemochromatosis - blood
Humans
Iron - blood
Magnetic Resonance Spectroscopy
Transferrin - metabolism
Ultrafiltration
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Summary:The nature of non-transferrin-bound iron in the plasma or serum of iron-overloaded hemochromatosis patients was studied by high performance liquid chromatography (HPLC) and high resolution nuclear magnetic resonance (NMR). 500-MHz proton Hahn spin-echo NMR spectra of plasma or serum, combined with the use of the iron chelator desferrioxamine, suggests complexation of iron ions with citrate and a possible involvement of acetate. Addition of FeCl3 to hemochromatosis samples broadened the NMR signals from citrate. HPLC analysis rigorously confirmed the presence of an iron-citrate complex in ultrafiltrates of plasma or serum studies with added FeCl3 or desferrioxamine supported this conclusion. It is proposed that non-transferrin-bound iron in the plasma of iron-overloaded patients exists largely as complexes with citrate and possibly also as ternary iron-citrate-acetate complexes. The presence of such complexes would account for the ability of non-transferrin-bound iron to be measurable by the bleomycin assay and for its rapid clearance from the circulation by the liver.
ISSN:0021-9258
1083-351X